Pectic polysaccharides from Radix Sophorae Tonkinensis exhibit significant antioxidant effects

Xin Ning, Ying Liu, Mengdi Jia, Qidi Wang, Ziyan Sun, Li Ji, Kevin H. Mayo, Yifa Zhou, Lin Sun

Research output: Contribution to journalArticlepeer-review

Abstract

Two pectic polysaccharides (WRSP-A2b and WRSP-A3a) have been obtained from Radix Sophorae Tonkinensis and comparatively investigated in terms of their physical properties and antioxidant activities. Monosaccharide composition, FT-IR, NMR and enzymatic analyses indicate that both WRSP-A2b (13.6 kDa) and WRSP-A3a (44.6 kDa) consist of homogalacturonan (HG), rhamnogalacturonan I (RG-I) and rhamnogalacturonan II (RG-II) domains, with mass ratios of 0.9:1.8:1 and 2.3:2.9:1, respectively. The RG-I domains were further purified and characterized. Results show that WRSP-A2b contains a highly branched RG-I domain, primarily substituted with α-(1→5)-linked arabinans, whereas WRSP-A3a contains a small branched RG-I domain mainly composed of β-(1→4)-linked galactan side chains. WRSP-A3a exhibits stronger antioxidant activity in scavenging different radicals than WRSP-A2b, a finding that may be due to its higher content of GalA residues and HG domains. Our results provide useful information for screening natural polysaccharide-based antioxidants from Radix Sophorae Tonkinensis.

Original languageEnglish (US)
Article number117925
JournalCarbohydrate Polymers
Volume262
DOIs
StatePublished - Jun 15 2021

Bibliographical note

Funding Information:
This work was supported by the National Science & Technology Major Project ?Key New Drug Creation and Manufacturing Program?, China (2019ZX09735001), National Natural Science Foundation of China (31770852), the Scientific and Technologic Foundation of Jilin Province (20200201004JC), and the Fundamental Research Funds for the Central Universities (2412020ZD001).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

  • Antioxidant activities
  • Pectin
  • Radix Sophorae Tonkinensis
  • Structural characterization

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