Phe-met-arg-phe-amide (FMRF-NH2) inhibits insulin and somatostatin secretion and anti-FMRF-NH2 sera detects pancreatic polypeptide cells in the rat islet

Robert L Sorenson; Cathrine A. Sasek; Robert P. Elde

doi:10.1016/0196-9781(84)90021-4

Phe-met-arg-phe-amide (FMRF-NH₂) inhibits insulin and somatostatin secretion and anti-FMRF-NH₂ sera detects pancreatic polypeptide cells in the rat islet

Robert L Sorenson, Cathrine A. Sasek, Robert P. Elde

Genetics, Cell Biology and Development (TMED)

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

FMRF-NH₂-like immunoreactivity was localized in the pancreatic polypeptide containing cells of the rat islet. FMRF-NH₂ was investigated with regard to its effect on insulin, somatostatin and glucagon secretion from the isolated perfused rat pancreas. FMRF-NH₂ (1 μM) significantly inhibited glucose stimulated (300 mg/dl) insulin release (p<0.005) and somatostatin release (p<0.01) from the isolated perfused pancreas. FMRF-NH₂ (1 and 10 μM) was without effect on glucagon secretion, either in low glucose (50 mg/dl), high glucose (300 mg/dl), or during arginine stimulation (5 mM). These findings indicate that these FMRF-NH₂ antisera recognize a substance in the pancreatic polypeptide cells of the islet which may be capable of modulating islet β and D cell activity.

Original language	English (US)
Pages (from-to)	777-782
Number of pages	6
Journal	Peptides
Volume	5
Issue number	4
DOIs	https://doi.org/10.1016/0196-9781(84)90021-4
State	Published - 1984

Bibliographical note

Funding Information:
The excellent technical assistance of Lynette Grouse, Marlys Rice and Jean Floyd is greatefully acknowledged. This research was supported by American Diabetes Association Minnesota Affiliate, lmmunonuclear Corporation and DA02148.

Keywords

FMRF-NH
Insulin
Islet
Somatostatin

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title = "Phe-met-arg-phe-amide (FMRF-NH2) inhibits insulin and somatostatin secretion and anti-FMRF-NH2 sera detects pancreatic polypeptide cells in the rat islet",

abstract = "FMRF-NH2-like immunoreactivity was localized in the pancreatic polypeptide containing cells of the rat islet. FMRF-NH2 was investigated with regard to its effect on insulin, somatostatin and glucagon secretion from the isolated perfused rat pancreas. FMRF-NH2 (1 μM) significantly inhibited glucose stimulated (300 mg/dl) insulin release (p<0.005) and somatostatin release (p<0.01) from the isolated perfused pancreas. FMRF-NH2 (1 and 10 μM) was without effect on glucagon secretion, either in low glucose (50 mg/dl), high glucose (300 mg/dl), or during arginine stimulation (5 mM). These findings indicate that these FMRF-NH2 antisera recognize a substance in the pancreatic polypeptide cells of the islet which may be capable of modulating islet β and D cell activity.",

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author = "Sorenson, {Robert L} and Sasek, {Cathrine A.} and Elde, {Robert P.}",

note = "Funding Information: The excellent technical assistance of Lynette Grouse, Marlys Rice and Jean Floyd is greatefully acknowledged. This research was supported by American Diabetes Association Minnesota Affiliate, lmmunonuclear Corporation and DA02148.",

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AU - Sorenson, Robert L

AU - Sasek, Cathrine A.

AU - Elde, Robert P.

N1 - Funding Information: The excellent technical assistance of Lynette Grouse, Marlys Rice and Jean Floyd is greatefully acknowledged. This research was supported by American Diabetes Association Minnesota Affiliate, lmmunonuclear Corporation and DA02148.

PY - 1984

Y1 - 1984

N2 - FMRF-NH2-like immunoreactivity was localized in the pancreatic polypeptide containing cells of the rat islet. FMRF-NH2 was investigated with regard to its effect on insulin, somatostatin and glucagon secretion from the isolated perfused rat pancreas. FMRF-NH2 (1 μM) significantly inhibited glucose stimulated (300 mg/dl) insulin release (p<0.005) and somatostatin release (p<0.01) from the isolated perfused pancreas. FMRF-NH2 (1 and 10 μM) was without effect on glucagon secretion, either in low glucose (50 mg/dl), high glucose (300 mg/dl), or during arginine stimulation (5 mM). These findings indicate that these FMRF-NH2 antisera recognize a substance in the pancreatic polypeptide cells of the islet which may be capable of modulating islet β and D cell activity.

AB - FMRF-NH2-like immunoreactivity was localized in the pancreatic polypeptide containing cells of the rat islet. FMRF-NH2 was investigated with regard to its effect on insulin, somatostatin and glucagon secretion from the isolated perfused rat pancreas. FMRF-NH2 (1 μM) significantly inhibited glucose stimulated (300 mg/dl) insulin release (p<0.005) and somatostatin release (p<0.01) from the isolated perfused pancreas. FMRF-NH2 (1 and 10 μM) was without effect on glucagon secretion, either in low glucose (50 mg/dl), high glucose (300 mg/dl), or during arginine stimulation (5 mM). These findings indicate that these FMRF-NH2 antisera recognize a substance in the pancreatic polypeptide cells of the islet which may be capable of modulating islet β and D cell activity.

KW - FMRF-NH

KW - Insulin

KW - Islet

KW - Somatostatin

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