TY - JOUR
T1 - Phenotypic and functional characterization of committed and primitive myeloid and lymphoid hematopoietic precursors in human fetal liver
AU - Roy, V.
AU - Miller, J. S.
AU - Verfaille, C. M.
PY - 1997
Y1 - 1997
N2 - We studied the phenotypic and functional properties of colony-forming cells (CFCs), primitive long-term culture initiating cells (LTC-ICs) and lymphoid precursors present in human fetal liver (FL) and compared these with their adult bone marrow (BM) counterparts. FL (7-14-week) cells were selected by fluorescence-activated cell sorting based on increasing CD34 antigen expression (34+, 34++, or 34+++), CD38 antigen expression (CD34(++/+++)CD38+, or CD38-), and HLA-DR antigen expression (CD34(++/+++)HLA-DR+ or HLA-DR-). 13 ± 0.6% of FL CD34-positive cells were 34+++. Significantly more FL CD34(++/+++) cells were CD38- (49 ± 2.4%) and HLA-DR- (72 ± 6.7%) than BM CD34++ cells (6.8 ± 0.7% CD38- and 13.3 ± 3.2% HLA-DR-). FL and BM CFCs were CD34(+/++), CD38+, and HLA-DR+. However, significantly more FL CFCs were erythroid (40%) than adult BM CFCs (15%), and FL colonies were larger (8111 ± 738 cells/CFC) than BM colonies (3466 ± 272 cells/CFC, p < 0.001). As is seen in adult BM, FL LTC-ICs were CD34(++/+++) CD38-. In contrast to BM LTC-ICs, FL LTC-ICs were almost exclusively CD34(++/+++) HLA-DR+. In addition, a single FL LTC-IC gave rise to >30 CFCs at 5 weeks compared with only 5 ± 0.9 CFCs per LTC-IC from BM. Finally, we demonstrate that the FL CD34(++/+++)/CD38-/HLA-DR+ population, which contains 3.7% LTC-ICs, also contains primitive lymphoid progenitors capable of differentiating into natural killer (NK) cells. In conclusion, the phenotype of primitive human FL progenitors such as LTC-IC and primitive NK progenitors is CD34(++/+++)/CD38-/HLA-DR+, suggesting that this population may contain FL hematopoietic stem cells. The phenotypic characterization of FL primitive LTC-ICs and NK progenitors will facilitate further studies of the functional properties of these progenitors.
AB - We studied the phenotypic and functional properties of colony-forming cells (CFCs), primitive long-term culture initiating cells (LTC-ICs) and lymphoid precursors present in human fetal liver (FL) and compared these with their adult bone marrow (BM) counterparts. FL (7-14-week) cells were selected by fluorescence-activated cell sorting based on increasing CD34 antigen expression (34+, 34++, or 34+++), CD38 antigen expression (CD34(++/+++)CD38+, or CD38-), and HLA-DR antigen expression (CD34(++/+++)HLA-DR+ or HLA-DR-). 13 ± 0.6% of FL CD34-positive cells were 34+++. Significantly more FL CD34(++/+++) cells were CD38- (49 ± 2.4%) and HLA-DR- (72 ± 6.7%) than BM CD34++ cells (6.8 ± 0.7% CD38- and 13.3 ± 3.2% HLA-DR-). FL and BM CFCs were CD34(+/++), CD38+, and HLA-DR+. However, significantly more FL CFCs were erythroid (40%) than adult BM CFCs (15%), and FL colonies were larger (8111 ± 738 cells/CFC) than BM colonies (3466 ± 272 cells/CFC, p < 0.001). As is seen in adult BM, FL LTC-ICs were CD34(++/+++) CD38-. In contrast to BM LTC-ICs, FL LTC-ICs were almost exclusively CD34(++/+++) HLA-DR+. In addition, a single FL LTC-IC gave rise to >30 CFCs at 5 weeks compared with only 5 ± 0.9 CFCs per LTC-IC from BM. Finally, we demonstrate that the FL CD34(++/+++)/CD38-/HLA-DR+ population, which contains 3.7% LTC-ICs, also contains primitive lymphoid progenitors capable of differentiating into natural killer (NK) cells. In conclusion, the phenotype of primitive human FL progenitors such as LTC-IC and primitive NK progenitors is CD34(++/+++)/CD38-/HLA-DR+, suggesting that this population may contain FL hematopoietic stem cells. The phenotypic characterization of FL primitive LTC-ICs and NK progenitors will facilitate further studies of the functional properties of these progenitors.
KW - Human fetal liver
KW - Long-term culture initiating cell
KW - Natural killer progenitors
KW - Phenotypic characterization
KW - Proliferative potential
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M3 - Article
C2 - 9168060
AN - SCOPUS:0031000430
SN - 0301-472X
VL - 25
SP - 387
EP - 394
JO - Experimental Hematology
JF - Experimental Hematology
IS - 5
ER -