Phorbol ester-induced changes in human leukemic pre-B lines

Nicoletta Sacchi; Tucker V. LeBien; Stephen Trost; Diego Breviario; F. J. Bollum

doi:10.1016/0008-8749(84)90077-7

Phorbol ester-induced changes in human leukemic pre-B lines

Nicoletta Sacchi, Tucker V. LeBien, Stephen Trost, Diego Breviario, F. J. Bollum

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin μ chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin μ chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant μ chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged μ chain genes.

Original language	English (US)
Pages (from-to)	65-73
Number of pages	9
Journal	Cellular Immunology
Volume	84
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(84)90077-7
State	Published - Mar 1984
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access

10.1016/0008-8749(84)90077-7

OpenUrl availability

Full text

Cite this

@article{7748e6ddeeea43fc885aff2a359514e7,

title = "Phorbol ester-induced changes in human leukemic pre-B lines",

abstract = "The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin μ chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin μ chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant μ chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged μ chain genes.",

author = "Nicoletta Sacchi and LeBien, {Tucker V.} and Stephen Trost and Diego Breviario and Bollum, {F. J.}",

year = "1984",

month = mar,

doi = "10.1016/0008-8749(84)90077-7",

language = "English (US)",

volume = "84",

pages = "65--73",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Phorbol ester-induced changes in human leukemic pre-B lines

AU - Sacchi, Nicoletta

AU - LeBien, Tucker V.

AU - Trost, Stephen

AU - Breviario, Diego

AU - Bollum, F. J.

PY - 1984/3

Y1 - 1984/3

N2 - The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin μ chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin μ chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant μ chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged μ chain genes.

AB - The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin μ chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin μ chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant μ chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged μ chain genes.

UR - http://www.scopus.com/inward/record.url?scp=0021355261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021355261&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(84)90077-7

DO - 10.1016/0008-8749(84)90077-7

M3 - Article

C2 - 6230159

AN - SCOPUS:0021355261

SN - 0008-8749

VL - 84

SP - 65

EP - 73

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Phorbol ester-induced changes in human leukemic pre-B lines

Abstract

UN SDGs

Access

OpenUrl availability

Other files and links

Fingerprint

Cite this