Phylogenetic analysis, genomic diversity and classification of M class gene segments of turkey reoviruses

Sunil K Mor, Douglas Marthaler, Harsha Verma, Tamer A. Sharafeldin, Naresh Jindal, Robert E Porter, Sagar M Goyal

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

From 2011 to 2014, 13 turkey arthritis reoviruses (TARVs) were isolated from cases of swollen hock joints in 2-18-week-old turkeys. In addition, two isolates from similar cases of turkey arthritis were received from another laboratory. Eight turkey enteric reoviruses (TERVs) isolated from fecal samples of turkeys were also used for comparison. The aims of this study were to characterize turkey reovirus (TRV) based on complete M class genome segments and to determine genetic diversity within TARVs in comparison to TERVs and chicken reoviruses (CRVs). Nucleotide (nt) cut off values of 84%, 83% and 85% for the M1, M2 and M3 gene segments were proposed and used for genotype classification, generating 5, 7, and 3 genotypes, respectively. Using these nt cut off values, we propose M class genotype constellations (GCs) for avian reoviruses. Of the seven GCs, GC1 and GC3 were shared between the TARVs and TERVs, indicating possible reassortment between turkey and chicken reoviruses. The TARVs and TERVs were divided into three GCs, and GC2 was unique to TARVs and TERVs. The proposed new GC approach should be useful in identifying reassortant viruses, which may ultimately be used in the design of a universal vaccine against both chicken and turkey reoviruses.

Original languageEnglish (US)
Pages (from-to)70-82
Number of pages13
JournalVeterinary Microbiology
Volume176
Issue number1-2
DOIs
StatePublished - Mar 23 2015

Bibliographical note

Funding Information:
This study was funded in part by a grant from the Rapid Agricultural Response Fund , established by the Minnesota legislature and administered by the University of Minnesota Agricultural Experiment Station. We thank Wendy Wiese and Jonathan Erber for technical assistance.

Publisher Copyright:
© 2015 Elsevier B.V.

Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.

Keywords

  • Genotypes
  • M class gene segments
  • Mutations
  • Phylogenetic analysis
  • Reassortment
  • Turkey arthritis reovirus

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