Phylogeny of bovine norovirus in Egypt based on VP2 gene

Fakry F. Mohamed, Gamelat K.F. Ktob, Mohamed E.A. Ismaeil, Ahmed A.H. Ali, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Bovine norovirus (BNoV) has emerged as a viral pathogen that causes a gastrointestinal illness and diarrhea in cattle. Despite its worldwide distribution, very little information is known about BNoV in Africa. In this study, BNoV was detected in 27.6% (8/29) of tested fecal materials, collected from sporadic cases of diarrheic calves, using the reverse transcription-polymerase chain reaction (RT-PCR) and primers that target RNA dependent RNA polymerase gene. Additionally, one primer pair was designed to flank the BNoV-VP2 (small capsid protein) gene for molecular analysis. Study VP2 sequences were phylogenetically-related to BNoV-GIII.2 (Newbury2-like) genotype, which is highly prevalent all over the world. However, they were separated within the cluster and one strain (41FR) grouped with recombinant GIII.P1/GIII.2 strains. Compared to reference VP2 sequences, 14 amino acid substitution mutations were found to be unique to our strains. The study confirms that BNoV is currently circulating among diarrheic calves of Egypt and also characterizes its ORF3 (VP2) genetically. The status of BNoV should be continuously evaluated in Egypt for effective prevention and control.

Original languageEnglish (US)
Pages (from-to)48-52
Number of pages5
JournalInternational Journal of Veterinary Science and Medicine
Issue number1
StatePublished - Apr 2018


  • Caliciviruses
  • Diarrhea
  • Gastroenteritis
  • Genogroups
  • ORF3
  • Phylogeny


Dive into the research topics of 'Phylogeny of bovine norovirus in Egypt based on VP2 gene'. Together they form a unique fingerprint.

Cite this