Abstract
Deep-sea hypersaline anoxic basins and other hypersaline environments contain abundant and diverse microbial life that has adapted to these extreme conditions. The bacterial Candidate Division KB1 represents one of several uncultured groups that have been consistently observed in hypersaline microbial diversity studies. Here we report the phylogeography of KB1, its phylogenetic relationships to Candidate Division OP1 Bacteria, and its potential metabolic and osmotic stress adaptations based on a partial single cell amplified genome of KB1 from Orca Basin, the largest hypersaline seafloor brine basin in the Gulf of Mexico. Our results are consistent with the hypothesis - previously developed based on 14C incorporation experiments with mixed-species enrichments from Mediterranean seafloor brines - that KB1 has adapted its proteins to elevated intracellular salinity, but at the same time KB1 apparently imports glycine betaine; this compatible solute is potentially not limited to osmoregulation but could also serve as a carbon and energy source.
Original language | English (US) |
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Article number | 1266 |
Journal | Frontiers in Microbiology |
Volume | 7 |
Issue number | AUG |
DOIs | |
State | Published - Aug 22 2016 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2016 Nigro, Hyde, MacGregor and Teske.
Keywords
- Candidate Division KB1
- Genome
- Hypersaline
- Orca Basin
- Phylogeography