Physicochemical characterization of hemicelluloses from bamboo (Phyllostachys pubescens Mazel) stem

Hong Peng, Na Wang, Zhengrong Hu, Ziping Yu, Yuhuan Liu, Jinsheng Zhang, Roger Ruan

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


In this paper, hemicelluloses from bamboo (Phyllostachys pubescens Mazel) stem aged six months were sequentially extracted with hot water, 2% KOH, and 5% NaOH. The water-soluble hemicelluloses H 1, and four alkali-soluble hemicellulosic fractions H 2, H 3, H 4 and H 5 were obtained, achieving a total yield of 59.60% based on the original hemicelluloses. Sugar composition analysis showed that the hemicelluloses were mainly composed of xylose (44.39-72.71%), arabinose (26.36-51.87%), ribose (0.93-2.72%), and uronic acid (0.29-5.27%). The structures of the hemicelluloses were determined to be mainly arabinoxylan, using the FT-IR and NMR techniques. The AFM images of fraction H 3 revealed a distribution of spherical nanoparticles with different sizes, while the fraction H 2 had helix rod and random coil feature. The SEM characterizations indicated that the fraction H 1 displayed spherical particles while the fraction H 3 had mainly flat particles at high magnification. Thermal stability was also analyzed using TG-DTG method and first-order kinetics model.

Original languageEnglish (US)
Pages (from-to)41-50
Number of pages10
JournalIndustrial Crops and Products
Issue number1
StatePublished - May 1 2012


  • Bamboo stem
  • Hemicelluloses
  • Morphological properties
  • Structure

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