Physicochemical properties and in vitro cytocompatibility of modified titanium surfaces prepared via micro-arc oxidation with different calcium concentrations

Sui Dan Wu, Hui Zhang, Xu Dong Dong, Cheng Yun Ning, Alex S.L. Fok, Yan Wang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Objective To explore the effect of calcium concentration in the electrolyte solution on the physicochemical properties and biocompatibility of coatings formed by micro-arc oxidation (MAO) on titanium surfaces. Methods The surfaces of pure titanium plates were modified by MAO in an electrolytic solution containing calcium acetate (CA; C 4 H 6 CaO 4 ) at concentrations of 0.05, 0.1, 0.2, or 0.3 M and β-glycerophosphate disodium salt pentahydrate (β-GP; C 3 H 7 Na 2 O 6 P·5H 2 O) at a fixed concentration of 0.02 M. Surface topography, elemental characteristics, phase composition, and roughness were investigated by scanning electron microscopy, energy-dispersive X-ray analysis, X-ray diffraction, and a surface roughness tester, respectively. To assess the cytocompatibility and osteoinductivity of the surfaces, MC3T3-E1 preosteoblasts were cultured on the surfaces in vitro, and cell morphology, adhesion, proliferation, and differentiation were observed. Results The porous MAO coating was composed primarily of TiO 2 rutile and anatase. The amount of TiO 2 rutile, the Ca/P ratio, and the surface roughness of the MAO coating increased with increasing CA concentration in the electrolyte solution. Ca 3 (PO 4 ) 2 , CaCO 3 , and CaTiO 3 were formed on MAO-treated surfaces prepared with CA concentrations of 0.2 and 0.3 M. Cell proliferation and differentiation increased with increasing CA concentration, with MC3T3-E1 cells exhibiting favorable morphologies for bone-implant integration. Conclusions MAO coating improves the surface characteristics and cytocompatibility of titanium for osseointegration. Higher CA concentration in the MAO electrolyte solution has a positive effect on the surface properties, chemical composition, and cell response.

Original languageEnglish (US)
Pages (from-to)347-355
Number of pages9
JournalApplied Surface Science
Volume329
DOIs
StatePublished - Feb 28 2015

Bibliographical note

Funding Information:
The authors greatly appreciate financial support from the National Natural Science Foundation of China ( 81141064 ), Natural Science Foundation of Guangdong Province ( S2013010016060 ) and National Basic Research Program of China ( 2012CB619100 ).

Keywords

  • Calcium concentration
  • Cytocompatibility
  • Micro-arc oxidation
  • Surface modification
  • Titanium

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