TY - JOUR
T1 - PilZ domain proteins bind cyclic diguanylate and regulate diverse processes in Vibrio cholerae
AU - Pratt, Jason T.
AU - Tamayo, Rita
AU - Tischler, Anna D.
AU - Camilli, Andrew
PY - 2007/4/27
Y1 - 2007/4/27
N2 - Cyclic diguanylate (c-di-GMP) is an allosteric activator and second messenger implicated in the regulation of a variety of biological processes in diverse bacteria. In Vibrio cholerae, c-di-GMP has been shown to inversely regulate biofilm-specific and virulence gene expression, suggesting that c-di-GMP signaling is important for the transition of V. cholerae from the environment to the host. However, the mechanism behind this regulation remains unknown. Recently, it was proposed that the PilZ protein domain represents a c-di-GMP-binding domain. Here we show that V. cholerae PilZ proteins bind c-di-GMP specifically and are involved in the regulation of biofilm formation, motility, and virulence. These findings confirm a role for PilZ proteins as c-di-GMP-sensing proteins within the c-di-GMP signaling network.
AB - Cyclic diguanylate (c-di-GMP) is an allosteric activator and second messenger implicated in the regulation of a variety of biological processes in diverse bacteria. In Vibrio cholerae, c-di-GMP has been shown to inversely regulate biofilm-specific and virulence gene expression, suggesting that c-di-GMP signaling is important for the transition of V. cholerae from the environment to the host. However, the mechanism behind this regulation remains unknown. Recently, it was proposed that the PilZ protein domain represents a c-di-GMP-binding domain. Here we show that V. cholerae PilZ proteins bind c-di-GMP specifically and are involved in the regulation of biofilm formation, motility, and virulence. These findings confirm a role for PilZ proteins as c-di-GMP-sensing proteins within the c-di-GMP signaling network.
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U2 - 10.1074/jbc.M611593200
DO - 10.1074/jbc.M611593200
M3 - Article
C2 - 17307739
AN - SCOPUS:34250358327
SN - 0021-9258
VL - 282
SP - 12860
EP - 12870
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 17
ER -