PKCι is a target of 7,8,4′-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression

Tae Gyu Lim; Yoon A. Kim; Jong Eun Kim; Sohee Baek; Sung Young Lee; Charles C. Lee; Hanyong Chen; Jong Rhan Kim; Jung Yeon Kwon; Ann M. Bode; Chang Yong Lee; Zigang Dong; Deok Kun Oh; Jun Seong Park; Ki Won Lee

doi:10.1111/exd.13375

PKCι is a target of 7,8,4′-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression

Tae Gyu Lim, Yoon A. Kim, Jong Eun Kim, Sohee Baek, Sung Young Lee, Charles C. Lee, Hanyong Chen, Jong Rhan Kim, Jung Yeon Kwon, Ann M. Bode, Chang Yong Lee, Zigang Dong, Deok Kun Oh, Jun Seong Park, Ki Won Lee

Hormel Institute

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The soy isoflavone daidzein is bioconverted to 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4′-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4′-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4′-THIF. Protein kinase C iota (PKCι) was suspected to be a direct target of 7,8,4′-THIF. The direct interaction between 7,8,4′-THIF and PKCι was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4′-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4′-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.

Original language	English (US)
Pages (from-to)	449-452
Number of pages	4
Journal	Experimental Dermatology
Volume	27
Issue number	5
DOIs	https://doi.org/10.1111/exd.13375
State	Published - May 2018

Bibliographical note

Funding Information:
This work was supported by the Korea Food Research Institute (E0164700-02), the Leap Research Program (2010-0029233) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT & Future Planning, and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio industry Technology Development Program (514004), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea.

Funding Information:
This work was supported by the Korea Food Research Institute (?0164700-02), the Leap Research Program (2010-0029233) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT & Future Planning, and the Korea Institute of Planning and ?valuation for Technology in Food, Agriculture, Forestry and Fisheries (IP?T) through Agri-Bio industry Technology Development Program (514004), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea.

Publisher Copyright:
© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Keywords

3D culture
7,8,4′-trihydroxyisoflavone
Protein kinase C iota
matrix metalloproteinase-1

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title = "PKCι is a target of 7,8,4′-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression",

abstract = "The soy isoflavone daidzein is bioconverted to 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4′-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4′-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4′-THIF. Protein kinase C iota (PKCι) was suspected to be a direct target of 7,8,4′-THIF. The direct interaction between 7,8,4′-THIF and PKCι was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4′-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4′-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.",

keywords = "3D culture, 7,8,4′-trihydroxyisoflavone, Protein kinase C iota, matrix metalloproteinase-1",

author = "Lim, {Tae Gyu} and Kim, {Yoon A.} and Kim, {Jong Eun} and Sohee Baek and Lee, {Sung Young} and Lee, {Charles C.} and Hanyong Chen and Kim, {Jong Rhan} and Kwon, {Jung Yeon} and Bode, {Ann M.} and Lee, {Chang Yong} and Zigang Dong and Oh, {Deok Kun} and Park, {Jun Seong} and Lee, {Ki Won}",

note = "Funding Information: This work was supported by the Korea Food Research Institute (E0164700-02), the Leap Research Program (2010-0029233) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT & Future Planning, and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio industry Technology Development Program (514004), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea. Funding Information: This work was supported by the Korea Food Research Institute (?0164700-02), the Leap Research Program (2010-0029233) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT & Future Planning, and the Korea Institute of Planning and ?valuation for Technology in Food, Agriculture, Forestry and Fisheries (IP?T) through Agri-Bio industry Technology Development Program (514004), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea. Publisher Copyright: {\textcopyright} 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

year = "2018",

month = may,

doi = "10.1111/exd.13375",

language = "English (US)",

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pages = "449--452",

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T1 - PKCι is a target of 7,8,4′-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression

AU - Lim, Tae Gyu

AU - Kim, Yoon A.

AU - Kim, Jong Eun

AU - Baek, Sohee

AU - Lee, Sung Young

AU - Lee, Charles C.

AU - Chen, Hanyong

AU - Kim, Jong Rhan

AU - Kwon, Jung Yeon

AU - Bode, Ann M.

AU - Lee, Chang Yong

AU - Dong, Zigang

AU - Oh, Deok Kun

AU - Park, Jun Seong

AU - Lee, Ki Won

N1 - Funding Information: This work was supported by the Korea Food Research Institute (E0164700-02), the Leap Research Program (2010-0029233) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT & Future Planning, and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agri-Bio industry Technology Development Program (514004), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea. Funding Information: This work was supported by the Korea Food Research Institute (?0164700-02), the Leap Research Program (2010-0029233) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT & Future Planning, and the Korea Institute of Planning and ?valuation for Technology in Food, Agriculture, Forestry and Fisheries (IP?T) through Agri-Bio industry Technology Development Program (514004), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea. Publisher Copyright: © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

PY - 2018/5

Y1 - 2018/5

N2 - The soy isoflavone daidzein is bioconverted to 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4′-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4′-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4′-THIF. Protein kinase C iota (PKCι) was suspected to be a direct target of 7,8,4′-THIF. The direct interaction between 7,8,4′-THIF and PKCι was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4′-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4′-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.

AB - The soy isoflavone daidzein is bioconverted to 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4′-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4′-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4′-THIF. Protein kinase C iota (PKCι) was suspected to be a direct target of 7,8,4′-THIF. The direct interaction between 7,8,4′-THIF and PKCι was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4′-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4′-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.

KW - 3D culture

KW - 7,8,4′-trihydroxyisoflavone

KW - Protein kinase C iota

KW - matrix metalloproteinase-1

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PKCι is a target of 7,8,4′-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression

Abstract

Bibliographical note

Keywords

UN SDGs

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