PKCι is a target of 7,8,4′-trihydroxyisoflavone for the suppression of UVB-induced MMP-1 expression

Tae Gyu Lim, Yoon A. Kim, Jong Eun Kim, Sohee Baek, Sung Young Lee, Charles C. Lee, Hanyong Chen, Jong Rhan Kim, Jung Yeon Kwon, Ann M. Bode, Chang Yong Lee, Zigang Dong, Deok Kun Oh, Jun Seong Park, Ki Won Lee

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The soy isoflavone daidzein is bioconverted to 7,8,4′-trihydroxyisoflavone (7,8,4′-THIF) by microorganisms. Here, we investigated the matrix metalloproteinase (MMP)-1 inhibitory properties of 7,8,4′-THIF that arise through the suppression of UVB-induced MMP-1 expression. 7,8,4′-THIF reduced UVB-induced MMP-1 expression at the transcriptional level in primary human dermal fibroblasts and inhibited UVB-induced transcriptional activity of AP-1, a major activator of MMP-1 expression. Additionally, it was observed that the mitogen-activated protein kinase (MAPK) pathway, a crucial signalling cascade for MMP-1 expression, was suppressed by 7,8,4′-THIF. Protein kinase C iota (PKCι) was suspected to be a direct target of 7,8,4′-THIF. The direct interaction between 7,8,4′-THIF and PKCι was confirmed using pull-down assays and immobilized metal ion affinity-based fluorescence polarization assays. Finally, we observed that 7,8,4′-THIF inhibited UVB-induced MMP-1 expression in a human skin equivalent model. Taken together, these results suggest that 7,8,4′-THIF, a bioconversion product of daidzein, suppresses UVB-induced MMP-1 expression.

Original languageEnglish (US)
Pages (from-to)449-452
Number of pages4
JournalExperimental Dermatology
Volume27
Issue number5
DOIs
StatePublished - May 2018

Bibliographical note

Funding Information:
This work was supported by the Korea Food Research Institute (?0164700-02), the Leap Research Program (2010-0029233) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT & Future Planning, and the Korea Institute of Planning and ?valuation for Technology in Food, Agriculture, Forestry and Fisheries (IP?T) through Agri-Bio industry Technology Development Program (514004), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) Republic of Korea.

Keywords

  • 3D culture
  • 7,8,4′-trihydroxyisoflavone
  • Protein kinase C iota
  • matrix metalloproteinase-1

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