Planar cell polarity genes Celsr1 and Vangl2 are necessary for kidney growth, differentiation, and rostrocaudal patterning

Hortensja Brzóska; Angela M. d'Esposito; Maria Kolatsi-Joannou; Vishal Patel; Peter Igarashi; Yunping Lei; Richard H. Finnell; Mark F. Lythgoe; Adrian S. Woolf; Eugenia Papakrivopoulou; David A. Long

doi:10.1016/j.kint.2016.07.011

Planar cell polarity genes Celsr1 and Vangl2 are necessary for kidney growth, differentiation, and rostrocaudal patterning

Hortensja Brzóska, Angela M. d'Esposito, Maria Kolatsi-Joannou, Vishal Patel, Peter Igarashi, Yunping Lei, Richard H. Finnell, Mark F. Lythgoe, Adrian S. Woolf, Eugenia Papakrivopoulou, David A. Long

Medicine - Renal and Hypertension Division

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35 Scopus citations

Abstract

The mammalian kidney contains nephrons comprising glomeruli and tubules joined to ureteric bud–derived collecting ducts. It has a characteristic bean-like shape, with near-complete rostrocaudal symmetry around the hilum. Here we show that Celsr1, a planar cell polarity (PCP) gene implicated in neural tube morphogenesis, is required for ureteric tree growth in early development and later in gestation prevents tubule overgrowth. We also found an interaction between Celsr1 and Vangl2 (another PCP gene) in ureteric tree growth, most marked in the caudal compartment of the kidneys from compound heterozygous mutant mice with a stunted rump. Furthermore, these genes together are required for the maturation of glomeruli. Interestingly, we demonstrated patients with CELSR1 mutations and spina bifida can have significant renal malformations. Thus, PCP genes are important in mammalian kidney development and have an unexpected role in rostrocaudal patterning during organogenesis.

Original language	English (US)
Pages (from-to)	1274-1284
Number of pages	11
Journal	Kidney international
Volume	90
Issue number	6
DOIs	https://doi.org/10.1016/j.kint.2016.07.011
State	Published - Dec 1 2016

Bibliographical note

Funding Information:
We thank Dr. Ian Smyth (Monash University) for providing Tree Surveyor software, UCL Biological Services for their assistance with animal experiments, and both Dr. Charlotte Dean (Imperial College London) and Professor Andrew Copp (UCL Great Ormond Street Institute of Child Health) for helpful discussions regarding this work. This work was supported by a PhD studentship from Kids Kidney Research (to EP and DAL), a UCL Bogue Research Fellowship (to HLB), a Wellcome Trust Postdoctoral Training Fellowship for MB/PhD graduates (grant no. 095949/Z/11/Z to EP), a Kidney Research UK Senior Non-Clinical Fellowship (grant no. SF1/2008 to DAL), a Medical Research Council New Investigator Award (grant no. MR/J003638/1 to DAL), and the National Institute for Health Research Biomedical Research Centre at Great Ormond Street Hospital for Children NHS Foundation Trust and University College London. ASW acknowledges support from the Medical Research Council (project grant no. MR/L002744/1) and the Manchester Biomedial Research Centre and the Manchester Academic Health Science Centre. VP and PI acknowledge support from the O’Brien Kidney Center at University of Texas Southwestern Medical Center (grant no. NIH P30-DK079328).

Publisher Copyright:
© 2016 International Society of Nephrology

Keywords

kidney development
planar cell polarity
renal malformations
rostrocaudal patterning
ureteric branching

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Brzóska, H., d'Esposito, A. M., Kolatsi-Joannou, M., Patel, V., Igarashi, P., Lei, Y., Finnell, R. H., Lythgoe, M. F., Woolf, A. S., Papakrivopoulou, E., & Long, D. A. (2016). Planar cell polarity genes Celsr1 and Vangl2 are necessary for kidney growth, differentiation, and rostrocaudal patterning. Kidney international, 90(6), 1274-1284. https://doi.org/10.1016/j.kint.2016.07.011

Brzóska, H, d'Esposito, AM, Kolatsi-Joannou, M, Patel, V, Igarashi, P, Lei, Y, Finnell, RH, Lythgoe, MF, Woolf, AS, Papakrivopoulou, E & Long, DA 2016, 'Planar cell polarity genes Celsr1 and Vangl2 are necessary for kidney growth, differentiation, and rostrocaudal patterning', Kidney international, vol. 90, no. 6, pp. 1274-1284. https://doi.org/10.1016/j.kint.2016.07.011

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abstract = "The mammalian kidney contains nephrons comprising glomeruli and tubules joined to ureteric bud–derived collecting ducts. It has a characteristic bean-like shape, with near-complete rostrocaudal symmetry around the hilum. Here we show that Celsr1, a planar cell polarity (PCP) gene implicated in neural tube morphogenesis, is required for ureteric tree growth in early development and later in gestation prevents tubule overgrowth. We also found an interaction between Celsr1 and Vangl2 (another PCP gene) in ureteric tree growth, most marked in the caudal compartment of the kidneys from compound heterozygous mutant mice with a stunted rump. Furthermore, these genes together are required for the maturation of glomeruli. Interestingly, we demonstrated patients with CELSR1 mutations and spina bifida can have significant renal malformations. Thus, PCP genes are important in mammalian kidney development and have an unexpected role in rostrocaudal patterning during organogenesis.",

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note = "Funding Information: We thank Dr. Ian Smyth (Monash University) for providing Tree Surveyor software, UCL Biological Services for their assistance with animal experiments, and both Dr. Charlotte Dean (Imperial College London) and Professor Andrew Copp (UCL Great Ormond Street Institute of Child Health) for helpful discussions regarding this work. This work was supported by a PhD studentship from Kids Kidney Research (to EP and DAL), a UCL Bogue Research Fellowship (to HLB), a Wellcome Trust Postdoctoral Training Fellowship for MB/PhD graduates (grant no. 095949/Z/11/Z to EP), a Kidney Research UK Senior Non-Clinical Fellowship (grant no. SF1/2008 to DAL), a Medical Research Council New Investigator Award (grant no. MR/J003638/1 to DAL), and the National Institute for Health Research Biomedical Research Centre at Great Ormond Street Hospital for Children NHS Foundation Trust and University College London. ASW acknowledges support from the Medical Research Council (project grant no. MR/L002744/1) and the Manchester Biomedial Research Centre and the Manchester Academic Health Science Centre. VP and PI acknowledge support from the O{\textquoteright}Brien Kidney Center at University of Texas Southwestern Medical Center (grant no. NIH P30-DK079328). Publisher Copyright: {\textcopyright} 2016 International Society of Nephrology",

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AU - Igarashi, Peter

AU - Lei, Yunping

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Planar cell polarity genes Celsr1 and Vangl2 are necessary for kidney growth, differentiation, and rostrocaudal patterning

Abstract

Bibliographical note

Keywords

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