Plasmid colIb contains an ssi signal close to the replication origin

Katsunori Tanaka; Tetsuya Sakai; Yoichi Honda; Hiroshi Hiasa; Hiroshi Sakai; Tohru Komano

doi:10.1016/0147-619X(91)90024-Q

Plasmid colIb contains an ssi signal close to the replication origin

Katsunori Tanaka, Tetsuya Sakai, Yoichi Honda, Hiroshi Hiasa, Hiroshi Sakai, Tohru Komano

Pharmacology

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Abstract

Taking advantage of the plaque morphology method, we identified a single-strand initiation (ssi) signal in plasmid pSM32, a mini-ColIb plasmid. This ssi signal was situated in the 350-nt HaeIII segment of the 1.8-kb S7 fragment, and located nearly 400 nt downstream of the origin of DNA replication. Introduction of the ssi signal into a mutant of filamentous phage M13 lacking ori_c resulted in restoration of phage growth and RFI DNA synthesis. Interestingly, DNA homology studies showed that the nucleotide sequence of the ssi signal was extremely homologous with that of the "G4-type" ssi signal in plasmid R100.

Original language	English (US)
Pages (from-to)	125-130
Number of pages	6
Journal	Plasmid
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/0147-619X(91)90024-Q
State	Published - Mar 1991

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abstract = "Taking advantage of the plaque morphology method, we identified a single-strand initiation (ssi) signal in plasmid pSM32, a mini-ColIb plasmid. This ssi signal was situated in the 350-nt HaeIII segment of the 1.8-kb S7 fragment, and located nearly 400 nt downstream of the origin of DNA replication. Introduction of the ssi signal into a mutant of filamentous phage M13 lacking oric resulted in restoration of phage growth and RFI DNA synthesis. Interestingly, DNA homology studies showed that the nucleotide sequence of the ssi signal was extremely homologous with that of the {"}G4-type{"} ssi signal in plasmid R100.",

author = "Katsunori Tanaka and Tetsuya Sakai and Yoichi Honda and Hiroshi Hiasa and Hiroshi Sakai and Tohru Komano",

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T1 - Plasmid colIb contains an ssi signal close to the replication origin

AU - Tanaka, Katsunori

AU - Sakai, Tetsuya

AU - Honda, Yoichi

AU - Hiasa, Hiroshi

AU - Sakai, Hiroshi

AU - Komano, Tohru

PY - 1991/3

Y1 - 1991/3

N2 - Taking advantage of the plaque morphology method, we identified a single-strand initiation (ssi) signal in plasmid pSM32, a mini-ColIb plasmid. This ssi signal was situated in the 350-nt HaeIII segment of the 1.8-kb S7 fragment, and located nearly 400 nt downstream of the origin of DNA replication. Introduction of the ssi signal into a mutant of filamentous phage M13 lacking oric resulted in restoration of phage growth and RFI DNA synthesis. Interestingly, DNA homology studies showed that the nucleotide sequence of the ssi signal was extremely homologous with that of the "G4-type" ssi signal in plasmid R100.

AB - Taking advantage of the plaque morphology method, we identified a single-strand initiation (ssi) signal in plasmid pSM32, a mini-ColIb plasmid. This ssi signal was situated in the 350-nt HaeIII segment of the 1.8-kb S7 fragment, and located nearly 400 nt downstream of the origin of DNA replication. Introduction of the ssi signal into a mutant of filamentous phage M13 lacking oric resulted in restoration of phage growth and RFI DNA synthesis. Interestingly, DNA homology studies showed that the nucleotide sequence of the ssi signal was extremely homologous with that of the "G4-type" ssi signal in plasmid R100.

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Plasmid colIb contains an ssi signal close to the replication origin

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