Abstract
To advance synthetic biology approaches that utilize S. oneidensis as host for biotechnology applications, we have investigated the variation in plasmid copy number of a modular vector set resulting from distinct origins of replication under different conditions. The replicons yielded a ≈9X-fold range for plasmid copy number variation in S. oneidensis (while the same origins yielded a ≈3X-fold range in Escherichia coli). This provides a sizeable range to control gene expression levels in S. oneidensis for synthetic biology applications. In addition, plasmid harboring the pBBR1 origin resulted in stable copy numbers in S. oneidensis under different conditions (mid-logarithmic, stationary, multi-plasmid). This may enable the realization of synthetic circuits in S. oneidensis where predictable, quantitative behavior is desired (in either single- or double-plasmid contexts).
Original language | English (US) |
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Title of host publication | 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society |
Subtitle of host publication | Enabling Innovative Technologies for Global Healthcare, EMBC 2020 |
Publisher | Institute of Electrical and Electronics Engineers Inc. |
Pages | 2372-2375 |
Number of pages | 4 |
ISBN (Electronic) | 9781728119908 |
DOIs | |
State | Published - Jul 2020 |
Event | 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society, EMBC 2020 - Montreal, Canada Duration: Jul 20 2020 → Jul 24 2020 |
Publication series
Name | Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS |
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Volume | 2020-July |
ISSN (Print) | 1557-170X |
Conference
Conference | 42nd Annual International Conferences of the IEEE Engineering in Medicine and Biology Society, EMBC 2020 |
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Country/Territory | Canada |
City | Montreal |
Period | 7/20/20 → 7/24/20 |
Bibliographical note
Publisher Copyright:© 2020 IEEE.