TY - JOUR
T1 - Plasmonic nanoholes in a multichannel microarray format for parallel kinetic assays and differential sensing
AU - Im, Hyungsoon
AU - Lesuffleur, Antoine
AU - Lindquist, Nathan C.
AU - Oh, Sang Hyun
PY - 2009/4/15
Y1 - 2009/4/15
N2 - We present nanohole arrays in a gold film integrated with a six-channel microfluidic chip for parallel measurements of molecular binding kinetics. Surface plasmon resonance effects in the nanohole arrays enable real-time, label-free measurements of molecular binding events in each channel, while adjacent negative reference channels can record measurement artifacts such as bulk solution index changes, temperature variations, or changing light absorption in the liquid. With the use of this platform, streptavidin - biotin specific binding kinetics are measured at various concentrations with negative controls. A high-density microarray of 252 biosensing pixels is also demonstrated with a packing density of 10 6 sensing elements/cm 2, which can potentially be coupled with a massively parallel array of microfluidic channels for protein microarray applications.
AB - We present nanohole arrays in a gold film integrated with a six-channel microfluidic chip for parallel measurements of molecular binding kinetics. Surface plasmon resonance effects in the nanohole arrays enable real-time, label-free measurements of molecular binding events in each channel, while adjacent negative reference channels can record measurement artifacts such as bulk solution index changes, temperature variations, or changing light absorption in the liquid. With the use of this platform, streptavidin - biotin specific binding kinetics are measured at various concentrations with negative controls. A high-density microarray of 252 biosensing pixels is also demonstrated with a packing density of 10 6 sensing elements/cm 2, which can potentially be coupled with a massively parallel array of microfluidic channels for protein microarray applications.
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U2 - 10.1021/ac802276x
DO - 10.1021/ac802276x
M3 - Article
C2 - 19284776
AN - SCOPUS:65249143347
SN - 0003-2700
VL - 81
SP - 2854
EP - 2859
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 8
ER -