Plasmonic nanoholes in a multichannel microarray format for parallel kinetic assays and differential sensing

Hyungsoon Im, Antoine Lesuffleur, Nathan C. Lindquist, Sang Hyun Oh

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

We present nanohole arrays in a gold film integrated with a six-channel microfluidic chip for parallel measurements of molecular binding kinetics. Surface plasmon resonance effects in the nanohole arrays enable real-time, label-free measurements of molecular binding events in each channel, while adjacent negative reference channels can record measurement artifacts such as bulk solution index changes, temperature variations, or changing light absorption in the liquid. With the use of this platform, streptavidin - biotin specific binding kinetics are measured at various concentrations with negative controls. A high-density microarray of 252 biosensing pixels is also demonstrated with a packing density of 10 6 sensing elements/cm 2, which can potentially be coupled with a massively parallel array of microfluidic channels for protein microarray applications.

Original languageEnglish (US)
Pages (from-to)2854-2859
Number of pages6
JournalAnalytical Chemistry
Volume81
Issue number8
DOIs
StatePublished - Apr 15 2009

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