Poached egg, a gene required in the soma to maintain germ cell viability in drosophila females

Phu V. Tran, Janette Pettus, Rod N. Nagoshi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In Drosophila, extensive interactions between the soma and germline are required for oogenesis. Interactions between the somatic ovary and follicle cells influence the differentiation and organization of the egg chamber. There is also evidence that the soma is needed to maintain germline viability. Little is known about the nature of these interactions or their genetic components. In this paper we identified a gene required in the soma for germline viability. Mutations in poached egg result in the induction of apoptosis in the nurse cells of stage 7 and later egg chambers. If oogenesis is arrested prior to vitellogenesis, poached egg -induced apoptosis does not occur. This indicates that poached egg function is dependent on some stage-specific process which coincides with the onset of vitellogenesis. We describe the genetic analysis of this newly identified gene, including the generation of new alleles and genetic interactions with the yolk protein complex.

Original languageEnglish (US)
Pages (from-to)21-35
Number of pages15
JournalInvertebrate Reproduction and Development
Volume39
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Bibliographical note

Funding Information:
We are grateful to members of the Nagoshi laboratory for helpful discussions. We thank the laboratories of Dr. Ganetzky and Dr. Jan for fly stocks and Dr. M. Bownes for the yolk protein antibody. This work was supported by grants from the National Institutes of Health and the Carver Foundation.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Apoptosis
  • Gametogenesis
  • Germline
  • Oogenesis

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