Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

E. Castellanos; A. Aranaz; B. Romero; L. De Juan; J. Alvarez; J. Bezos; S. Rodríguez; K. Stevenson; A. Mateos; L. Domínguez

doi:10.1128/JCM.01411-07

Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

E. Castellanos, A. Aranaz, B. Romero, L. De Juan, J. Alvarez, J. Bezos, S. Rodríguez, K. Stevenson, A. Mateos, L. Domínguez

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Abstract

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

Original language	English (US)
Pages (from-to)	3439-3442
Number of pages	4
Journal	Journal of clinical microbiology
Volume	45
Issue number	10
DOIs	https://doi.org/10.1128/JCM.01411-07
State	Published - Oct 2007
Externally published	Yes

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abstract = "The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.",

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AU - Castellanos, E.

AU - Aranaz, A.

AU - Romero, B.

AU - De Juan, L.

AU - Alvarez, J.

AU - Bezos, J.

AU - Rodríguez, S.

AU - Stevenson, K.

AU - Mateos, A.

AU - Domínguez, L.

PY - 2007/10

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AB - The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

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Polymorphisms in gyrA and gyrB genes among Mycobacterium avium subsp. paratuberculosis type I, II, and III isolates

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