TY - JOUR
T1 - Polysaccharide isolated from Poria cocos sclerotium induces NF-κB/ Rel activation and iNOS expression in murine macrophages
AU - Lee, Kun Yeong
AU - Jeon, Young Jin
N1 - Funding Information:
This study was supported by research funds from Chosun University, 2001.
PY - 2003/10
Y1 - 2003/10
N2 - We show that PCSC, a polysaccharide isolated from the sclerotium of Poria cocos with 1% sodium carbonate, significantly induces nitric oxide (NO) production and inducible NO synthase (iNOS) transcription through the activation of nuclear factor-κB/Rel (NF-κB/Rel). In vivo administration of PCSC induced NO production by peritoneal macrophages of B6C3F1 mice. PCSC also dose-dependently induced the production of NO in isolated mouse peritoneal macrophages and RAW 264.7, a murine macrophage-like cell line. Moreover, iNOS protein and mRNA transcription were strongly induced by PCSC in RAW 264.7 cells. To further investigate the mechanism responsible for the induction of iNOS gene expression, we investigated the effect of PCSC on the activation of transcription factors including NF-κB/Rel and Oct, whose binding sites were located in the promoter of iNOS gene. Treatment of RAW 264.7 cells with PCSC produced strong induction of NF-κB/Rel-dependent reporter gene expression, whereas Oct-dependent gene expression was not affected by PCSC. DNA binding activity of NF-κB/Rel was significantly induced by PCSC, and this effect was mediated through the degradation of IκBα. In conclusion, we demonstrate that PCSC stimulates macrophages to express iNOS gene through the activation of NF-κB/Rel.
AB - We show that PCSC, a polysaccharide isolated from the sclerotium of Poria cocos with 1% sodium carbonate, significantly induces nitric oxide (NO) production and inducible NO synthase (iNOS) transcription through the activation of nuclear factor-κB/Rel (NF-κB/Rel). In vivo administration of PCSC induced NO production by peritoneal macrophages of B6C3F1 mice. PCSC also dose-dependently induced the production of NO in isolated mouse peritoneal macrophages and RAW 264.7, a murine macrophage-like cell line. Moreover, iNOS protein and mRNA transcription were strongly induced by PCSC in RAW 264.7 cells. To further investigate the mechanism responsible for the induction of iNOS gene expression, we investigated the effect of PCSC on the activation of transcription factors including NF-κB/Rel and Oct, whose binding sites were located in the promoter of iNOS gene. Treatment of RAW 264.7 cells with PCSC produced strong induction of NF-κB/Rel-dependent reporter gene expression, whereas Oct-dependent gene expression was not affected by PCSC. DNA binding activity of NF-κB/Rel was significantly induced by PCSC, and this effect was mediated through the degradation of IκBα. In conclusion, we demonstrate that PCSC stimulates macrophages to express iNOS gene through the activation of NF-κB/Rel.
KW - Macrophages
KW - NF-κB/Rel
KW - Poria cocos
KW - iNOS
UR - http://www.scopus.com/inward/record.url?scp=0041926655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041926655&partnerID=8YFLogxK
U2 - 10.1016/S1567-5769(03)00113-9
DO - 10.1016/S1567-5769(03)00113-9
M3 - Article
C2 - 12946432
AN - SCOPUS:0041926655
SN - 1567-5769
VL - 3
SP - 1353
EP - 1362
JO - International Immunopharmacology
JF - International Immunopharmacology
IS - 10-11
ER -