Porcine myelomonocytic markers: Summary of the Second International Swine CD Workshop

J. Domínguez, A. Ezquerra, F. Alonso, K. McCullough, A. Summerfield, A. Bianchi, R. J. Zwart, Y. B. Kim, F. Blecha, S. Eicher, M. Murtaugh, M. Pampusch, K. Burger

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Forty five mAbs submitted to the Second International Swine CD workshop were analyzed by six different laboratories for their possible reactivity with porcine myelomonocytic cells using flow cytometry and immunohistochemistry. As a result of these analyses, a new swine workshop cluster, SWC9, composed of two mAbs that recognize an antigen selectively expressed mature macrophages, was defined. In addition, several mAbs were identified, allowing the differentiation of granulocytes from monocytes/macrophages, or monocytes from macrophages. Further work is required to identify the antigen recognized by these mAbs. Nevertheless, they should already prove useful for the identification of different stages in the macrophage maturation/differentiation, and will certainly aid analyses on the complexity of the mononuclear phagocyte system in the pig. Finally, the cross-reactivity of three anti-human CD14 mAbs with porcine myelomonocytic ceils was established in this workshop.

Original languageEnglish (US)
Pages (from-to)329-341
Number of pages13
JournalVeterinary immunology and immunopathology
Volume60
Issue number3-4
DOIs
StatePublished - Jan 30 1998

Keywords

  • CD14 mAbs
  • Flow cytometry
  • Immunohistochemistry
  • Porcine myelomonocytic markers

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