Abstract
We present a capture-based approach for bisulfite-converted DNA that allows interrogation of predefined genomic locations, allowing quantitative and qualitative assessments of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) at CG dinucleotides and in non-CG contexts (CHG, CHH) in mammalian and plant genomes. We show the technique works robustly and reproducibly using as little as 500 ng of starting DNA, with results correlating well with whole genome bisulfite sequencing data, and demonstrate that human DNA can be tested in samples contaminated with microbial DNA. This targeting approach will allow cell type-specific designs to maximize the value of 5mC and 5hmC sequencing.
Original language | English (US) |
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Article number | e81 |
Journal | Nucleic acids research |
Volume | 43 |
Issue number | 12 |
DOIs | |
State | Published - Jul 13 2015 |
Bibliographical note
Publisher Copyright:© The Author(s) 2015.