Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm

Naoyuki Kataoka, Jeongsik Yong, V. Narry Kim, Francisco Velazquez, Robert A. Perkinson, Fan Wang, Gideon Dreyfuss

Research output: Contribution to journalArticlepeer-review

279 Scopus citations

Abstract

We describe a novel RNA binding protein, Y14, a predominantly nuclear nucleocytoplasmic shuttling protein. Interestingly, Y14 associates preferentially with mRNAs produced by splicing but not with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Y14 associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Splicing of a single intron is sufficient for Y14 association. Y14-containing nuclear complexes are different from general hnRNP complexes. They contain hnRNP proteins and several unique proteins including the mRNA export factor TAP. Thus, Y14 defines novel intermediate in the pathway of gene expression, postsplicing nuclear preexport mRNPs, and newly exported cytoplasmic mRNPs, whose composition is established by splicing. These findings suggest that pre-mRNA splicing imprints mRNA with a unique set of proteins that persists in the cytoplasm and thereby communicates the history of the transcript.

Original languageEnglish (US)
Pages (from-to)673-682
Number of pages10
JournalMolecular Cell
Volume6
Issue number3
DOIs
StatePublished - Sep 2000

Fingerprint Dive into the research topics of 'Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm'. Together they form a unique fingerprint.

Cite this