Predicting the response of the deep-ocean microbiome to geochemical perturbations by hydrothermal vents

Daniel C. Reed, John A. Breier, Houshuo Jiang, Karthik Anantharaman, Christopher A. Klausmeier, Brandy M. Toner, Cathrine Hancock, Kevin Speer, Andreas M. Thurnherr, Gregory J. Dick

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Submarine hydrothermal vents perturb the deep-ocean microbiome by injecting reduced chemical species into the water column that act as an energy source for chemosynthetic organisms. These systems thus provide excellent natural laboratories for studying the response of microbial communities to shifts in marine geochemistry. The present study explores the processes that regulate coupled microbial-geochemical dynamics in hydrothermal plumes by means of a novel mathematical model, which combines thermodynamics, growth and reaction kinetics, and transport processes derived from a fluid dynamics model. Simulations of a plume located in the ABE vent field of the Lau basin were able to reproduce metagenomic observations well and demonstrated that the magnitude of primary production and rate of autotrophic growth are largely regulated by the energetics of metabolisms and the availability of electron donors, as opposed to kinetic parameters. Ambient seawater was the dominant source of microbes to the plume and sulphur oxidisers constituted almost 90% of the modelled community in the neutrally-buoyant plume. Data from drifters deployed in the region allowed the different time scales of metabolisms to be cast in a spatial context, which demonstrated spatial succession in the microbial community. While growth was shown to occur over distances of tens of kilometers, microbes persisted over hundreds of kilometers. Given that high-temperature hydrothermal systems are found less than 100 km apart on average, plumes may act as important vectors between different vent fields and other environments that are hospitable to similar organisms, such as oil spills and oxygen minimum zones.

Original languageEnglish (US)
Pages (from-to)1857-1869
Number of pages13
JournalISME Journal
Volume9
Issue number8
DOIs
StatePublished - Aug 23 2015

Bibliographical note

Funding Information:
This work is funded by Gordon and Betty Moore Foundation grant GBMF2609 and NSF grant OCE 1038006 to GJD, NSF grants OCE-0241785 and OCE-1231803 to KS, NSF grant OCE-1037991 to BMT, and OCE-1038055 to JAB and HJ. We thank the Captain and crew of the R/V Thomas G Thompson as well as the crew of the ROV Jason II. Thanks to Drs AL Reysenbach, M Tivey, C Fisher, P Girguis, G Luther and the Eastern Lau Spreading Center 2009 scientific parties for allowing us to participate in their cruises (NSF grants: OCE-0424953, OCE-02040985, OCE-0728391, OCE-0752469 and OCE-0751839). Also, we thank Drs Sheri White and Jason Sylvan for assistance with sampling. Nucleotide sequences are available in the NCBI Sequence Read Archive, accession numbers SRX511269, SRX511304 and SRX511275 (Supplementary Table S4). These data have been previously published by Anantharaman et al. (2014). The custom databases, blastx results and reads that hit each gene referenced in this study are available at the following URL along with the model code: www.earth.lsa. umich.edu/geomicrobiology/Lau.html

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