TY - JOUR
T1 - Preferential tissue localization of bovine γδ T cell subsets defined by anti-T cell receptor for antigen antibodies
AU - Wilson, E.
AU - Walcheck, B.
AU - Davis, W. C.
AU - Jutila, M. A.
N1 - Funding Information:
This study was supported by funds from USDA NRI 96-35204-3580, Animal Health, NIH S10RR11877 and the Murdock Foundation.
PY - 1998/11
Y1 - 1998/11
N2 - Clonal and oligoclonal populations of γδ T cells, with respect to the expression of T cell receptors for antigen (Tcr), have been shown to localize in normal and inflamed tissues. The mechanisms responsible for the tissue- selective accumulation of these subsets are still not known. γδ T cells are the predominant T cell subset in newborn calves, making this animal a useful model to study these cells. However, molecular markers defining tissue- specific bovine γδ T cell subsets are only now being developed. In this report, we describe three new anti-bovine γδ Tcr mAbs: GD3.8, GD197 and GD3.1, which provide useful tools to study these cells. GD3.8 recognized virtually all γδ T cells in the blood; whereas GD3.1 and GD197 recognized mutually exclusive as well as overlapping subsets. Using these three mAbs, four separate subsets of γδ T cells were defined: subset 1 (GD3.8 +, GD3.1+, GD197-); subset 2 (GD3.8+, GD3.1-, GD197+); subset 3 (GD3.8+, GD3.1+, GD197+); and subset 4 (GD3.8+, GD3.1-, GD197-). Subset 4 constituted a minor population in the blood; however, it predominated in the spleen and, in some cases, represented a 300% increase over blood levels. The percentage of GD3.1-positive γδ T cells was found to be increased in experimentally inflamed lymph nodes, suggesting that subset 1 cells may be preferentially retained in or recruited to sites of inflammation. Some subset 4 cells also exhibited a decreased ability to respond to PHA. These studies demonstrate that bovine γδ T cell, Tcr-defined subsets, exhibit unique accumulation and activation characteristics that may provide clues to their function and regulation.
AB - Clonal and oligoclonal populations of γδ T cells, with respect to the expression of T cell receptors for antigen (Tcr), have been shown to localize in normal and inflamed tissues. The mechanisms responsible for the tissue- selective accumulation of these subsets are still not known. γδ T cells are the predominant T cell subset in newborn calves, making this animal a useful model to study these cells. However, molecular markers defining tissue- specific bovine γδ T cell subsets are only now being developed. In this report, we describe three new anti-bovine γδ Tcr mAbs: GD3.8, GD197 and GD3.1, which provide useful tools to study these cells. GD3.8 recognized virtually all γδ T cells in the blood; whereas GD3.1 and GD197 recognized mutually exclusive as well as overlapping subsets. Using these three mAbs, four separate subsets of γδ T cells were defined: subset 1 (GD3.8 +, GD3.1+, GD197-); subset 2 (GD3.8+, GD3.1-, GD197+); subset 3 (GD3.8+, GD3.1+, GD197+); and subset 4 (GD3.8+, GD3.1-, GD197-). Subset 4 constituted a minor population in the blood; however, it predominated in the spleen and, in some cases, represented a 300% increase over blood levels. The percentage of GD3.1-positive γδ T cells was found to be increased in experimentally inflamed lymph nodes, suggesting that subset 1 cells may be preferentially retained in or recruited to sites of inflammation. Some subset 4 cells also exhibited a decreased ability to respond to PHA. These studies demonstrate that bovine γδ T cell, Tcr-defined subsets, exhibit unique accumulation and activation characteristics that may provide clues to their function and regulation.
KW - Blast cell
KW - Homing
KW - Spleen
KW - Tcr
KW - γδ T cell
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U2 - 10.1016/S0165-2478(98)00077-7
DO - 10.1016/S0165-2478(98)00077-7
M3 - Article
C2 - 9865600
AN - SCOPUS:0032416690
SN - 0165-2478
VL - 64
SP - 39
EP - 44
JO - Immunology Letters
JF - Immunology Letters
IS - 1
ER -