Preferential tissue localization of bovine γδ T cell subsets defined by anti-T cell receptor for antigen antibodies

E. Wilson, B. Walcheck, W. C. Davis, M. A. Jutila

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


Clonal and oligoclonal populations of γδ T cells, with respect to the expression of T cell receptors for antigen (Tcr), have been shown to localize in normal and inflamed tissues. The mechanisms responsible for the tissue- selective accumulation of these subsets are still not known. γδ T cells are the predominant T cell subset in newborn calves, making this animal a useful model to study these cells. However, molecular markers defining tissue- specific bovine γδ T cell subsets are only now being developed. In this report, we describe three new anti-bovine γδ Tcr mAbs: GD3.8, GD197 and GD3.1, which provide useful tools to study these cells. GD3.8 recognized virtually all γδ T cells in the blood; whereas GD3.1 and GD197 recognized mutually exclusive as well as overlapping subsets. Using these three mAbs, four separate subsets of γδ T cells were defined: subset 1 (GD3.8 +, GD3.1+, GD197-); subset 2 (GD3.8+, GD3.1-, GD197+); subset 3 (GD3.8+, GD3.1+, GD197+); and subset 4 (GD3.8+, GD3.1-, GD197-). Subset 4 constituted a minor population in the blood; however, it predominated in the spleen and, in some cases, represented a 300% increase over blood levels. The percentage of GD3.1-positive γδ T cells was found to be increased in experimentally inflamed lymph nodes, suggesting that subset 1 cells may be preferentially retained in or recruited to sites of inflammation. Some subset 4 cells also exhibited a decreased ability to respond to PHA. These studies demonstrate that bovine γδ T cell, Tcr-defined subsets, exhibit unique accumulation and activation characteristics that may provide clues to their function and regulation.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalImmunology Letters
Issue number1
StatePublished - Nov 1 1998


  • Blast cell
  • Homing
  • Spleen
  • Tcr
  • γδ T cell

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