Prenatal viral infection causes alterations in nNOS expression in developing mouse brains

S H Fatemi, Adolfo E. Cuadra, Esam E El-Fakahany, Robert W. Sidwell, Paul Thuras

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Epidemiological evidence points to prenatal viral infection being responsible for some forms of schizophrenia and autism. We hypothesized that prenatal human influenza viral infection in day 9 pregnant mice may cause changes in the levels of neuronal nitric oxide synthase (nNOS), an important molecule involved in synaptogenesis and excitotoxicity, in neonatal brains. Brains from 35- and 56-day-old mice were prepared for SDS-gel electrophoresis and Western blotting using polyclonal anti nNOS antibody. Quantification of nNOS showed time and region-dependent changes in the levels of nNOS protein. Mean rostral brain area value from prenatally infected animals showed a significant (p = 0.067) increase of 147% in nNOS levels at 35 days postnatally, with an eventual 29% decrease on day 56. Middle and caudal brain areas showed reductions in nNOS in experimental mice at 35 and 56 days, with a significant 27% decrease in nNOS in the middle segment of day 56 brains (p = 0.016). Significant interactions were found between group membership and brain area (Wilks lambda = 0.440, F(2.9) = 5.72, p = 0.025); there was also a significant interaction between brain area, group and age (Wilks lambda = 0.437, F(2.9) = 5.79, p = 0.024). These results provide further support for the notion that prenatal viral infection affects brain development adversely via the pathological involvement of nNOS expression. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish (US)
Pages (from-to)1493-1496
Number of pages4
JournalNeuroreport
Volume11
Issue number7
DOIs
StatePublished - May 15 2000

Keywords

  • Human influenza
  • Neuronal nitric oxide synthase
  • Neurotoxicity
  • Prenatal
  • Schizophrenia

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