Preparation of amino acid mixtures for cell-free expression systems

Filippo Caschera, Vincent Noireaux

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Here we present a procedure for preparing amino acid mixtures-having both the desired composition and a physiological pH-at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all Escherichia coli cell-free expression system. Our method is fast, easy to execute, and economically advantageous compared to expensive commercial kits, making it useful for high-throughput experiments, incorporation of nonstandard amino acids, and cell-free metabolic engineering.

Original languageEnglish (US)
Pages (from-to)40-43
Number of pages4
JournalBioTechniques
Volume58
Issue number1
DOIs
StatePublished - Jan 1 2015

Keywords

  • Amino acid distribution
  • Amino acid mixtures
  • Cell-free transcription-translation
  • pH

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