Presence of cu-type (Nirk) and cd1-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.

Jeonghwan Jang; Naoaki Ashida; Ayaaki Kai; Kazuo Isobe; Tomoyasu Nishizawa; Shigeto Otsuka; Akira Yokota; Keishi Senoo; Satoshi Ishii

doi:10.1264/jsme2.ME18039

Presence of cu-type (Nirk) and cd₁-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.

Jeonghwan Jang, Naoaki Ashida, Ayaaki Kai, Kazuo Isobe, Tomoyasu Nishizawa, Shigeto Otsuka, Akira Yokota, Keishi Senoo, Satoshi Ishii

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd₁-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1^T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1^T (=JCM 18858^T=KCTC 62391^T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

Original language	English (US)
Pages (from-to)	326-331
Number of pages	6
Journal	Microbes and environments
Volume	33
Issue number	3
DOIs	https://doi.org/10.1264/jsme2.ME18039
State	Published - 2018

Bibliographical note

Publisher Copyright:
© 2018, Japanese Society of Microbial Ecology. All rights reserved.

Keywords

Bradyrhizobium
Denitrification
Genome
New species

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10.1264/jsme2.ME18039

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title = "Presence of cu-type (Nirk) and cd1-type (nirs) nitrite reductase genes in the denitrifying bacterium bradyrhizobium nitroreducens sp. nov.",

abstract = "Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd1-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1T (=JCM 18858T=KCTC 62391T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.",

keywords = "Bradyrhizobium, Denitrification, Genome, New species",

author = "Jeonghwan Jang and Naoaki Ashida and Ayaaki Kai and Kazuo Isobe and Tomoyasu Nishizawa and Shigeto Otsuka and Akira Yokota and Keishi Senoo and Satoshi Ishii",

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AU - Jang, Jeonghwan

AU - Ashida, Naoaki

AU - Kai, Ayaaki

AU - Isobe, Kazuo

AU - Nishizawa, Tomoyasu

AU - Otsuka, Shigeto

AU - Yokota, Akira

AU - Senoo, Keishi

AU - Ishii, Satoshi

PY - 2018

Y1 - 2018

N2 - Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd1-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1T (=JCM 18858T=KCTC 62391T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

AB - Nitrite reductase is a key enzyme for denitrification. There are two types of nitrite reductases: copper-containing NirK and cytochrome cd1-containing NirS. Most denitrifiers possess either nirK or nirS, although a few strains been reported to possess both genes. We herein report the presence of nirK and nirS in the soil-denitrifying bacterium Bradyrhizobium sp. strain TSA1T. Both nirK and nirS were identified and actively transcribed under denitrification conditions. Based on physiological, chemotaxonomic, and genomic properties, strain TSA1T (=JCM 18858T=KCTC 62391T) represents a novel species within the genus Bradyrhizobium, for which we propose the name Bradyrhizobium nitroreducens sp. nov.

KW - Bradyrhizobium

KW - Denitrification

KW - Genome

KW - New species

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