TY - JOUR
T1 - Preventing bacterial contamination and proliferation during the harvest, storage, and feeding of fresh bovine colostrum
AU - Stewart, S.
AU - Godden, S.
AU - Bey, R.
AU - Rapnicki, P.
AU - Fetrow, J.
AU - Farnsworth, R.
AU - Scanlon, M.
AU - Arnold, Y.
AU - Clow, L.
AU - Mueller, K.
AU - Ferrouillet, C.
N1 - Funding Information:
This study was funded by the Minnesota Rapid Agricultural Response Fund . The authors would like to express their extreme appreciation to Jim Lewis, Dan Priestler, and the owners of Emerald II Dairy (the TMF) for their cooperation with the study.
PY - 2005/7
Y1 - 2005/7
N2 - The objectives of this study were to identify control points for bacterial contamination of bovine colostrum during the harvesting and feeding processes, and to describe the effects of refrigeration and use of potassium sorbate preservative on bacteria counts in stored fresh colostrum. For objective 1, first-milking colostrum samples were collected aseptically directly from the mammary glands of 39 cows, from the milking bucket, and from the esophageal feeder tube. For objective 2, 15-mL aliquots of colostrum were collected from the milking bucket and allocated to 1 of 4 treatment groups: 1) refrigeration, 2) ambient temperature, 3) refrigeration with potassium sorbate preservative, and 4) ambient temperature with potassium sorbate preservative. Subsamples from each treatment group were collected after 24, 48, and 96 h of storage. All samples underwent bacteriological culture for total plate count and coliform count. Bacteria counts were generally low or zero in colostrum collected directly from the gland [mean (SD) log10 cfu/mLudder = 1.44 (1.45)]. However, significant bacterial contamination occurred during the harvest process [mean (SD) log10 cfu/mLbucket = 4.99 (1.95)]. No additional bacterial contamination occurred between the bucket and the esophageal feeder tube. Storing colostrum at warm ambient temperatures resulted in the most rapid increase in bacteria counts, followed by intermediate rates of growth in nonpreserved refrigerated samples or preserved samples stored at ambient temperature. The most effective treatment studied was the use of potassium sorbate preservative in refrigerated samples, for which total plate count and total coliform counts dropped significantly and then remained constant during the 96-h storage period.
AB - The objectives of this study were to identify control points for bacterial contamination of bovine colostrum during the harvesting and feeding processes, and to describe the effects of refrigeration and use of potassium sorbate preservative on bacteria counts in stored fresh colostrum. For objective 1, first-milking colostrum samples were collected aseptically directly from the mammary glands of 39 cows, from the milking bucket, and from the esophageal feeder tube. For objective 2, 15-mL aliquots of colostrum were collected from the milking bucket and allocated to 1 of 4 treatment groups: 1) refrigeration, 2) ambient temperature, 3) refrigeration with potassium sorbate preservative, and 4) ambient temperature with potassium sorbate preservative. Subsamples from each treatment group were collected after 24, 48, and 96 h of storage. All samples underwent bacteriological culture for total plate count and coliform count. Bacteria counts were generally low or zero in colostrum collected directly from the gland [mean (SD) log10 cfu/mLudder = 1.44 (1.45)]. However, significant bacterial contamination occurred during the harvest process [mean (SD) log10 cfu/mLbucket = 4.99 (1.95)]. No additional bacterial contamination occurred between the bucket and the esophageal feeder tube. Storing colostrum at warm ambient temperatures resulted in the most rapid increase in bacteria counts, followed by intermediate rates of growth in nonpreserved refrigerated samples or preserved samples stored at ambient temperature. The most effective treatment studied was the use of potassium sorbate preservative in refrigerated samples, for which total plate count and total coliform counts dropped significantly and then remained constant during the 96-h storage period.
KW - Bacterial contamination
KW - Colostrum
KW - Preservative
KW - Storage
UR - http://www.scopus.com/inward/record.url?scp=22944458522&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22944458522&partnerID=8YFLogxK
U2 - 10.3168/jds.S0022-0302(05)72933-7
DO - 10.3168/jds.S0022-0302(05)72933-7
M3 - Article
C2 - 15956318
AN - SCOPUS:22944458522
SN - 0022-0302
VL - 88
SP - 2571
EP - 2578
JO - Journal of Dairy Science
JF - Journal of Dairy Science
IS - 7
ER -