Abstract
Black soybean seed coat is a rich source of polyphenols reported to have various physiological functions. The present study investigated the potential protective effectsof polyphenolic extracts from black soybean seed coat on mutagen-induced DNA damage in Salmonella typhimurium. Results from the Ames test using Salmonella stains revealed that black soybean seed coat extract (BE) at 5000 μg/plate showed neither mutagenicity nor cytotoxicity. In contrast, BE, its component cyanidin 3-glucoside (C3G), or procyanidin-rich BE (PC-rich BE) dose-dependently inhibited B(a)P- and 4NQO-induced mutagenesis in the rank order: C3G > BE > PC-rich BE. At 5 μM/plate, C3G and procyanidins, the major polyphenols in BE, inhibited B(a)P-induced mutagenesis in TA 98 and TA 100by 28 - 59%. They also inhibited 4NQOinduced mutagenesis by 26 - 56%. Collectively, we found that C3G and procyanidins, which are the main active compounds in BE, effectivelyprotected against DNA mutagenic damage in Salmonella typhimurium.
Original language | English (US) |
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Pages (from-to) | 685-690 |
Number of pages | 6 |
Journal | Food Science and Technology Research |
Volume | 19 |
Issue number | 4 |
DOIs | |
State | Published - 2013 |
Externally published | Yes |
Keywords
- Ames test
- Black soybean seed coat extract
- Cyanidin 3-glucoside
- DNA damage
- Procyanidins