Probing subtle fluorescence dynamics in cellular proteins by streak camera based fluorescence lifetime imaging microscopy

R. V. Krishnan; Eva Biener; Jian Hua Zhang; Robert Heckel; Brian Herman

doi:10.1063/1.1630154

Probing subtle fluorescence dynamics in cellular proteins by streak camera based fluorescence lifetime imaging microscopy

R. V. Krishnan, Eva Biener, Jian Hua Zhang, Robert Heckel, Brian Herman

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

An overview is given on the possibility to measure fluorescence dynamics of cellular proteins with high spatial/temporal resolution. It is shown that such measurement capability yield useful guidelines in designing proper controls for quantitative live cell imaging such as FRET microscopy and in situ pH imaging.

Original language	English (US)
Pages (from-to)	4658-4660
Number of pages	3
Journal	Applied Physics Letters
Volume	83
Issue number	22
DOIs	https://doi.org/10.1063/1.1630154
State	Published - Dec 1 2003

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abstract = "An overview is given on the possibility to measure fluorescence dynamics of cellular proteins with high spatial/temporal resolution. It is shown that such measurement capability yield useful guidelines in designing proper controls for quantitative live cell imaging such as FRET microscopy and in situ pH imaging.",

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AU - Krishnan, R. V.

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AU - Herman, Brian

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Probing subtle fluorescence dynamics in cellular proteins by streak camera based fluorescence lifetime imaging microscopy

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