Probing the microstructure of hydrogels using fluorescence recovery after photobleaching

Santripti Khandai, Ronald A Siegel, Sidhartha S. Jena

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Diffusion of labeled BSA in polyacrylamide hydrogels was studied with variation of polymer and crosslinker concentration in order to probe the microstructure of the hydrogels. With the rise in polymer concentration beyond a certain threshold, single exponential recovery shifted to double exponential, suggesting structural changes in the hydrogel matrix. A similar shift from single to double diffusive behavior was seen on increasing crosslinker concentration at a given polymer concentration.

Original languageEnglish (US)
Title of host publicationSolid State Physics - Proceedings of the 57th DAE Solid State Physics Symposium 2012
Pages134-135
Number of pages2
Volume1512
DOIs
StatePublished - Mar 15 2013
Event57th DAE Solid State Physics Symposium 2012 - Bombay, Mumbai, India
Duration: Dec 3 2012Dec 7 2012

Other

Other57th DAE Solid State Physics Symposium 2012
CountryIndia
CityBombay, Mumbai
Period12/3/1212/7/12

Keywords

  • Diffusion
  • FRAP
  • Hydrogels
  • Structure
  • Transport dynamics

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