Probing the microstructure of hydrogels using fluorescence recovery after photobleaching

Santripti Khandai; Ronald A Siegel; Sidhartha S. Jena

doi:10.1063/1.4790947

Probing the microstructure of hydrogels using fluorescence recovery after photobleaching

Santripti Khandai, Ronald A Siegel, Sidhartha S. Jena

Pharmaceutics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Diffusion of labeled BSA in polyacrylamide hydrogels was studied with variation of polymer and crosslinker concentration in order to probe the microstructure of the hydrogels. With the rise in polymer concentration beyond a certain threshold, single exponential recovery shifted to double exponential, suggesting structural changes in the hydrogel matrix. A similar shift from single to double diffusive behavior was seen on increasing crosslinker concentration at a given polymer concentration.

Original language	English (US)
Title of host publication	Solid State Physics - Proceedings of the 57th DAE Solid State Physics Symposium 2012
Pages	134-135
Number of pages	2
Volume	1512
DOIs	https://doi.org/10.1063/1.4790947
State	Published - Mar 15 2013
Event	57th DAE Solid State Physics Symposium 2012 - Bombay, Mumbai, India Duration: Dec 3 2012 → Dec 7 2012

Other

Other	57th DAE Solid State Physics Symposium 2012
Country/Territory	India
City	Bombay, Mumbai
Period	12/3/12 → 12/7/12

Keywords

Diffusion
FRAP
Hydrogels
Structure
Transport dynamics

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title = "Probing the microstructure of hydrogels using fluorescence recovery after photobleaching",

abstract = "Diffusion of labeled BSA in polyacrylamide hydrogels was studied with variation of polymer and crosslinker concentration in order to probe the microstructure of the hydrogels. With the rise in polymer concentration beyond a certain threshold, single exponential recovery shifted to double exponential, suggesting structural changes in the hydrogel matrix. A similar shift from single to double diffusive behavior was seen on increasing crosslinker concentration at a given polymer concentration.",

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AU - Siegel, Ronald A

AU - Jena, Sidhartha S.

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Y1 - 2013/3/15

N2 - Diffusion of labeled BSA in polyacrylamide hydrogels was studied with variation of polymer and crosslinker concentration in order to probe the microstructure of the hydrogels. With the rise in polymer concentration beyond a certain threshold, single exponential recovery shifted to double exponential, suggesting structural changes in the hydrogel matrix. A similar shift from single to double diffusive behavior was seen on increasing crosslinker concentration at a given polymer concentration.

AB - Diffusion of labeled BSA in polyacrylamide hydrogels was studied with variation of polymer and crosslinker concentration in order to probe the microstructure of the hydrogels. With the rise in polymer concentration beyond a certain threshold, single exponential recovery shifted to double exponential, suggesting structural changes in the hydrogel matrix. A similar shift from single to double diffusive behavior was seen on increasing crosslinker concentration at a given polymer concentration.

KW - Diffusion

KW - FRAP

KW - Hydrogels

KW - Structure

KW - Transport dynamics

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EP - 135

BT - Solid State Physics - Proceedings of the 57th DAE Solid State Physics Symposium 2012

T2 - 57th DAE Solid State Physics Symposium 2012

Y2 - 3 December 2012 through 7 December 2012

ER -

Probing the microstructure of hydrogels using fluorescence recovery after photobleaching

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