Probiotic Lactobacillus rhamnosus GG mono-association suppresses human rotavirus-induced autophagy in the gnotobiotic piglet intestine

Shaoping Wu, Lijuan Yuan, Yongguo Zhang, Fangning Liu, Guohua Li, Ke Wen, Jacob Kocher, Xingdong Yang, Jun Sun

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Background: Human rotavirus (HRV) is the most important cause of severe diarrhea in infants and young children. Probiotic Lactobacillus rhamnosus GG (LGG) reduces rotavirus infection and diarrhea. However, the molecular mechanisms of LGG-mediated protection from rotavirus infection are poorly understood. Autophagy plays an essential role in responses to microbial pathogens. However, the role of autophagy in HRV infection and LGG treatment is unknown. We hypothesize that rotavirus gastroenteritis activates autophagy and that LGG suppresses virus-induced autophagy and prevents intestinal damage in infected piglets. Methods. We used LGG feeding to combat viral gastroenteritis in the gnotobiotic pig model of virulent HRV infection. Results: We found that LGG feeding did not increase autophagy, whereas virus infection induced autophagy in the piglet intestine. Virus infection increased the protein levels of the autophagy markers ATG16L1 and Beclin-1 and the autophagy regulator mTOR. LGG treatment during viral gastroenteritis reduced autophagy marker expression to normal levels, induced apoptosis and partially prevented virus-induced tissue damage. Conclusion: Our study provides new insights into virus-induced autophagy and LGG suppression of uncontrolled autophagy and intestinal injury. A better understanding of the antiviral activity of LGG will lead to novel therapeutic strategies for infant infectious diseases.

Original languageEnglish (US)
Article number22
JournalGut Pathogens
Volume5
Issue number1
DOIs
StatePublished - 2013

Keywords

  • Apoptosis
  • Autophagy
  • Diarrhea
  • Gnotobiotic pig
  • Infectious disease
  • Intestinal inflammation
  • Intestinal injury
  • Lactobacillus rhamnosus GG
  • Probiotics
  • Rotavirus

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