Procedures to Evaluate Inflammatory and Pathological Changes During Allergic Airway Inflammation

Savita P. Rao; Stephanie Rastle-Simpson; Mythili Dileepan; Srirama Rao

doi:10.1007/978-1-0716-1001-5_16

Procedures to Evaluate Inflammatory and Pathological Changes During Allergic Airway Inflammation

Savita P. Rao, Stephanie Rastle-Simpson, Mythili Dileepan, Srirama Rao

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Cellular inflammation, with elevated levels of Th1/Th2 cytokines, airway mucus hypersecretion, and thickening of the airway smooth muscle, are characteristic features of the allergic lung. Assessment of pathophysiological changes in allergic lungs serves as an important tool to determine disease progression and understand the underlying mechanisms of pathogenesis. This can be achieved by evaluating the lung tissue for inflammation and airway structural changes along with the measurement of important pro-inflammatory mediators such as Th1/Th2 cytokines and eotaxins. This chapter describes procedures to histologically evaluate inflammatory and pathological changes observed during allergic airway inflammation using lung tissue from mice.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	217-236
Number of pages	20
DOIs	https://doi.org/10.1007/978-1-0716-1001-5_16
State	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2223
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Airway mucus secretion
Airway smooth muscle hypertrophy
Allergic inflammation
Cytokines
Fibrosis
Histology
Lung tissue
Th1
Th2
Tissue lysis

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-1001-5_16

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title = "Procedures to Evaluate Inflammatory and Pathological Changes During Allergic Airway Inflammation",

abstract = "Cellular inflammation, with elevated levels of Th1/Th2 cytokines, airway mucus hypersecretion, and thickening of the airway smooth muscle, are characteristic features of the allergic lung. Assessment of pathophysiological changes in allergic lungs serves as an important tool to determine disease progression and understand the underlying mechanisms of pathogenesis. This can be achieved by evaluating the lung tissue for inflammation and airway structural changes along with the measurement of important pro-inflammatory mediators such as Th1/Th2 cytokines and eotaxins. This chapter describes procedures to histologically evaluate inflammatory and pathological changes observed during allergic airway inflammation using lung tissue from mice.",

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note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2021",

doi = "10.1007/978-1-0716-1001-5_16",

language = "English (US)",

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AU - Rastle-Simpson, Stephanie

AU - Dileepan, Mythili

AU - Rao, Srirama

PY - 2021

Y1 - 2021

N2 - Cellular inflammation, with elevated levels of Th1/Th2 cytokines, airway mucus hypersecretion, and thickening of the airway smooth muscle, are characteristic features of the allergic lung. Assessment of pathophysiological changes in allergic lungs serves as an important tool to determine disease progression and understand the underlying mechanisms of pathogenesis. This can be achieved by evaluating the lung tissue for inflammation and airway structural changes along with the measurement of important pro-inflammatory mediators such as Th1/Th2 cytokines and eotaxins. This chapter describes procedures to histologically evaluate inflammatory and pathological changes observed during allergic airway inflammation using lung tissue from mice.

AB - Cellular inflammation, with elevated levels of Th1/Th2 cytokines, airway mucus hypersecretion, and thickening of the airway smooth muscle, are characteristic features of the allergic lung. Assessment of pathophysiological changes in allergic lungs serves as an important tool to determine disease progression and understand the underlying mechanisms of pathogenesis. This can be achieved by evaluating the lung tissue for inflammation and airway structural changes along with the measurement of important pro-inflammatory mediators such as Th1/Th2 cytokines and eotaxins. This chapter describes procedures to histologically evaluate inflammatory and pathological changes observed during allergic airway inflammation using lung tissue from mice.

KW - Airway mucus secretion

KW - Airway smooth muscle hypertrophy

KW - Allergic inflammation

KW - Cytokines

KW - Fibrosis

KW - Histology

KW - Lung tissue

KW - Th1

KW - Th2

KW - Tissue lysis

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M3 - Chapter

C2 - 33226598

AN - SCOPUS:85096779924

T3 - Methods in Molecular Biology

SP - 217

EP - 236

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Procedures to Evaluate Inflammatory and Pathological Changes During Allergic Airway Inflammation

Abstract

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