Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

Sun Young Kang, Bo Min Kim, Kyung Taek Heo, Jae Hyuk Jang, Won Gon Kim, Young Soo Hong

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.

Original languageEnglish (US)
Pages (from-to)2104-2111
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume27
Issue number12
DOIs
StatePublished - Dec 2017

Bibliographical note

Funding Information:
This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant No. PJ0110842017), Rural Development Administration, Republic of Korea.

Publisher Copyright:
© 2017 by The Korean Society for Microbiology and Biotechnology.

Keywords

  • Artificial biosynthesis
  • Caffeoyl-HSL
  • Feruloyl-HSL
  • Phenylacetyl homoserine lactone

Fingerprint

Dive into the research topics of 'Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway'. Together they form a unique fingerprint.

Cite this