Production of human immune interferon by recombinant mammalian cells cultivated on microcarriers

A. L. Smiley, W. ‐S Hu, D. I.C. Wang

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Recombinant Chinese hamster ovary (rCHO) cells were cultivated on microcarriers for the production of human immune (Gamma) interferon. The effect of basal medium, serum, and microcarrier concentration on interferon production was investigated. The specific interferon productivity in the post‐confluent stage was similar to that in the growth stage. Control of the pH results in a significant improvement in the volumetric interferon production. The volumetric production rate of interferon by these rCHO cells did not decrease after one month of cultivation on microcarriers.

Original languageEnglish (US)
Pages (from-to)1182-1190
Number of pages9
JournalBiotechnology and Bioengineering
Volume33
Issue number9
DOIs
StatePublished - Apr 5 1989

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