Production of methanethiol and volatile sulfur compounds by the archaeon "Ferroplasma acidarmanus"

David J. Baumler, Kai Foong Hung, Kwang Cheol Jeong, Charles W. Kaspar

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13 Scopus citations

Abstract

Acidophiles are typically isolated from sulfate-rich ecological niches yet the role of sulfur metabolism in their growth and survival is poorly defined. Studies of heterotrophically grown "Ferroplasma acidarmanus" showed that its growth requires a minimum of 100 mM of a sulfate-containing salt. Headspace gas analyses by GC/MS determined that the volatile sulfur compound emitted by active "F. acidarmanus" cultures is methanethiol. In "F. acidarmanus" cultures grown either heterotrophically or chemolithotrophically, methanethiol was produced constitutively. Radiotracer studies with 35S-labeled methionine, cysteine, and sulfate showed that all three were used in methanethiol production. Additionally, 3H-labeled methionine was incorporated into methanethiol and was probably used as a methyl-group donor. Methanethiol production in whole cell lysates supplied with SO 3 2- indicated that NADPH-dependant sulfite reductase and methyltransferase activities were present. Cell lysates also contained enzymatic activity for methionine-γ-lyase that cleaved the side chain of either methionine to form methanethiol or cysteine to produce H2S. Since methanethiol was detected from the degradation of cysteine, it is likely that sulfide was methylated by a thiol methyltransferase. Collectively, these data demonstrate that "F. acidarmanus" produces methanethiol through the metabolism of methionine, cysteine, or sulfate. This is the first report of a methanethiol-producing acidophile, thus identifying a new contributor to the global sulfur cycle.

Original languageEnglish (US)
Pages (from-to)841-851
Number of pages11
JournalExtremophiles
Volume11
Issue number6
DOIs
StatePublished - Nov 2007

Bibliographical note

Funding Information:
Acknowledgments We thank Dr. Yeonhwa Park, Jayne Storkson, Karen Albright, Wei Liu, Dr. Mike W. Pariza (Department of Food Microbiology and Toxicology, University of Wisconsin-Madison), and Lea McMartin, Brad Pierce, and Dr. Brian G. Fox (Department of Biochemistry, University of Wisconsin-Madison) for assistance with GC/MS studies. This work was supported by the Michael and Winona Foster Wisconsin Distinguished Graduate Fellowship (D.J.B) and in part by NSF-LexEn Grant MCB-9978205, and the University of Wisconsin-Madison, College of Agricultural and Life Sciences.

Keywords

  • Cysteine
  • Methanethiol
  • Methionine
  • Sulfate
  • Sulfite reductase
  • Thiol methyltransferase

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