Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7

Odayme Quesada, Brittney Gurda, Lakshmanan Govindasamy, Robert McKenna, Erik Kohlbrenner, George Aslanidi, Sergei Zolotukhin, Nicholas Muzyczka, Mavis Agbandje-Mckenna

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to ∼3.0 Å resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 Å in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.

Original languageEnglish (US)
Pages (from-to)1073-1076
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume63
Issue number12
DOIs
StatePublished - Nov 30 2007
Externally publishedYes

Keywords

  • Adeno-associated virus serotype 7

Fingerprint Dive into the research topics of 'Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7'. Together they form a unique fingerprint.

Cite this