TY - JOUR
T1 - Profiling DNA methylation by bisulfite genomic sequencing
T2 - problems and solutions.
AU - Liu, Liang
AU - Wylie, Rebecca C.
AU - Hansen, Nathaniel J.
AU - Andrews, Lucy G.
AU - Tollefsbol, Trygve O.
PY - 2004
Y1 - 2004
N2 - The surge of interest in DNA methylation during the last two decades has triggered an urgent need for an effective method to detect the methylation status of the cytosines in the genome. Bisulfite genomic sequencing is the most attractive choice so far for many laboratories. Various protocols have been established, but difficulties are often encountered, particularly by individuals who have limited experience in this field. This analysis presents a simple protocol that has consistently worked well in our laboratory. Discussions of potential technical problems and corresponding solutions are also included to facilitate the reproducibility of this protocol.
AB - The surge of interest in DNA methylation during the last two decades has triggered an urgent need for an effective method to detect the methylation status of the cytosines in the genome. Bisulfite genomic sequencing is the most attractive choice so far for many laboratories. Various protocols have been established, but difficulties are often encountered, particularly by individuals who have limited experience in this field. This analysis presents a simple protocol that has consistently worked well in our laboratory. Discussions of potential technical problems and corresponding solutions are also included to facilitate the reproducibility of this protocol.
UR - http://www.scopus.com/inward/record.url?scp=5444257510&partnerID=8YFLogxK
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U2 - 10.1385/1-59259-828-5:169
DO - 10.1385/1-59259-828-5:169
M3 - Article
C2 - 15273411
AN - SCOPUS:5444257510
SN - 1064-3745
VL - 287
SP - 169
EP - 179
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -