We have examined the influence of recombinant, human IL-3 on normal and leukemic B cell precursors. CD10/CALLA+ leukemic B cell precursors, the pre-B cell line BLIN-1, and surface IgM- B cell precursors isolated from fetal bone marrow all responded to IL-3. This IL-3 response was dose-dependent and could be abrogated by a rabbit anti-IL-3 antiserum. Binding studies using radiolabeled Il-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a K(D) of 150 pM. We conclude that normal and leukemic human B cell precursors express functional IL-3 receptors, extending the biologic range of this hematopoietin to the lymphoid lineage.
|Original language||English (US)|
|Number of pages||6|
|State||Published - 1989|