Prolonged treatment of peanut-allergic mice with bortezomib significantly reduces serum anti-peanut IgE but does not affect allergic symptoms

Daphne M. Moutsoglou, Stephen C. Dreskin

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Background: Anti-peanut immunoglobulin E (anti-Pn IgE) can persist throughout life, suggesting that this condition could be maintained by long-lived antibody-secreting cells (ASCs). To determine the role of long-lived ASCs, peanut-allergic mice underwent prolonged treatment with the proteasome inhibitor, bortezomib (Bz). Methods: Intravenous Bz was given twice weekly for 21 weeks to peanut-allergic mice. During treatment, serum anti-Pn IgE was measured, and the mice were rechallenged at the end of treatment. Cell populations were measured, and Pn-specific IgG, total IgG, and total IgE ASCs were enumerated in the bone marrow (BM) and spleen (SPL). Results: Prolonged treatment with Bz significantly reduced serum anti-Pn IgE and IgG1 but did not affect symptoms following challenge with Pn, even in mice with undetectable serum anti-Pn IgE. Numbers of CD138+ cells were significantly reduced in the BM but were unaffected in the SPL. Unexpectedly, Bz did not affect numbers of Pn-specific IgG, total IgG, or total IgE ASCs in either the BM or SPL. Conclusions: Cells that maintain long-lived serum anti-Pn IgE are sensitive to Bz. However, prolonged depletion of serum Pn-specific IgE does not result in a decrease of symptoms following challenge with Pn.

Original languageEnglish (US)
Pages (from-to)257-261
Number of pages5
JournalInternational Archives of Allergy and Immunology
Volume170
Issue number4
DOIs
StatePublished - Nov 1 2016

Bibliographical note

Publisher Copyright:
© 2016 S. Karger AG, Basel. Copyright: All rights reserved.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Anaphylaxis
  • Antibody-secreting cell
  • Immunoglobulin E
  • Mast cell
  • Proteasome inhibitor

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