TY - JOUR
T1 - Prostaglandins as reducing agents
T2 - A model of adenylate cyclase activation?
AU - Peterson, D. A.
AU - Kelly, B.
AU - Mehta, N.
AU - Gerrard, J. M.
PY - 1988/11
Y1 - 1988/11
N2 - It has been suggested that adenylate cyclase activation involves reduction of a disulfide linkage. Prostaglandin E1 (PGE1), prostaglandin E2 (PGE2), prostaglandin I2 (PGI2) and prostaglandin F2α (PGF2α) were tested for their ability to act as reducing agents with either cytochrome c, or the disulfide 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), the latter with a catalytic amount of ferric chloride. PGE1, PGE2, and PGI2 significantly reduced cytochrome c while PGF2α did not. PGE1, PGE2 and PGI2 reduced DTNB while PGF2α did not. The results are consistent with the postulate that prostaglandins which are effective in activating adenylate cyclase can act as reducing agents and might be involved in reductive activation of adenylate cyclase.
AB - It has been suggested that adenylate cyclase activation involves reduction of a disulfide linkage. Prostaglandin E1 (PGE1), prostaglandin E2 (PGE2), prostaglandin I2 (PGI2) and prostaglandin F2α (PGF2α) were tested for their ability to act as reducing agents with either cytochrome c, or the disulfide 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), the latter with a catalytic amount of ferric chloride. PGE1, PGE2, and PGI2 significantly reduced cytochrome c while PGF2α did not. PGE1, PGE2 and PGI2 reduced DTNB while PGF2α did not. The results are consistent with the postulate that prostaglandins which are effective in activating adenylate cyclase can act as reducing agents and might be involved in reductive activation of adenylate cyclase.
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U2 - 10.1016/0090-6980(88)90012-3
DO - 10.1016/0090-6980(88)90012-3
M3 - Article
C2 - 2853423
AN - SCOPUS:0024226687
SN - 0090-6980
VL - 36
SP - 667
EP - 671
JO - Prostaglandins
JF - Prostaglandins
IS - 5
ER -