Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora

Christèle Humblot; Evelyne Lhoste; Siegfried Knasmüller; Karine Gloux; Aurélia Bruneau; Martine Bensaada; José Durao; Sylvie Rabot; Claude Andrieux; Fekadu Kassie

doi:10.1016/j.jchromb.2003.11.018

Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora

Christèle Humblot, Evelyne Lhoste, Siegfried Knasmüller, Karine Gloux, Aurélia Bruneau, Martine Bensaada, José Durao, Sylvie Rabot, Claude Andrieux, Fekadu Kassie

Veterinary Clinical Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

We investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes (reduction ranged from 74% with inulin to 39% with Brussels sprouts) and colonocytes (reduction ranged from 68% with inulin to 56% with Brussels sprouts). This chemoprotective effect correlated with the induction of hepatic UDP-glucuronosyl transferase following Brussels sprouts consumption, and with alterations of bacterial metabolism in the distal gut (acidification, increase of butyrate proportion, decrease of β-glucuronidase activity) following inulin consumption.

Original language	English (US)
Pages (from-to)	231-237
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	802
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2003.11.018
State	Published - Mar 25 2004

Bibliographical note

Funding Information:
This study was financially supported by the EC, under the specific RTD program “Quality of Life and Management of Living Resources”, QLK1-CT99-01197, entitled “Heterocyclic Amines in Cooked Foods—Role in Human Health”. The authors thank Rosa Durao for animal care, Sandrine Bruel, Solène Garrido, Françoise Popot and Pierre Vaissade for their technical help during the experiments. Many thanks also to Dr. Ruud Verkerk (University of Wageningen, The Netherlands) for the gift of freeze–dried Brussels sprouts and to Dr. Jan Van Loo (Orafti) for the RAFTILOSE ® Synergy 1.

Keywords

2-Amino-3-methylimidazo[4,5-f] quinoline
Brassica
Fermented milk
Genotoxicity
Oligosaccharides

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Humblot, C., Lhoste, E., Knasmüller, S., Gloux, K., Bruneau, A., Bensaada, M., Durao, J., Rabot, S., Andrieux, C., & Kassie, F. (2004). Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 802(1), 231-237. https://doi.org/10.1016/j.jchromb.2003.11.018

Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora. / Humblot, Christèle; Lhoste, Evelyne; Knasmüller, Siegfried et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 802, No. 1, 25.03.2004, p. 231-237.

Research output: Contribution to journal › Article › peer-review

Humblot, C, Lhoste, E, Knasmüller, S, Gloux, K, Bruneau, A, Bensaada, M, Durao, J, Rabot, S, Andrieux, C & Kassie, F 2004, 'Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 802, no. 1, pp. 231-237. https://doi.org/10.1016/j.jchromb.2003.11.018

Humblot C, Lhoste E, Knasmüller S, Gloux K, Bruneau A, Bensaada M et al. Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2004 Mar 25;802(1):231-237. doi: 10.1016/j.jchromb.2003.11.018

Humblot, Christèle ; Lhoste, Evelyne ; Knasmüller, Siegfried et al. / Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat : Role of xenobiotic metabolising enzymes and intestinal microflora. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2004 ; Vol. 802, No. 1. pp. 231-237.

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note = "Funding Information: This study was financially supported by the EC, under the specific RTD program “Quality of Life and Management of Living Resources”, QLK1-CT99-01197, entitled “Heterocyclic Amines in Cooked Foods—Role in Human Health”. The authors thank Rosa Durao for animal care, Sandrine Bruel, Sol{\`e}ne Garrido, Fran{\c c}oise Popot and Pierre Vaissade for their technical help during the experiments. Many thanks also to Dr. Ruud Verkerk (University of Wageningen, The Netherlands) for the gift of freeze–dried Brussels sprouts and to Dr. Jan Van Loo (Orafti) for the RAFTILOSE {\textregistered} Synergy 1. ",

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AU - Humblot, Christèle

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Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora

Abstract

Bibliographical note

Keywords

Access

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