Protein half-lives of calmodulin and the plasma membrane Ca-ATPase in rat brain

Deborah A. Ferrington, Xingyu Chen, Arkadi G. Krainev, Elias K. Michaelis, Diana J. Bigelow

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20 Scopus citations


We report the half-lives for two proteins involved in the regulation of intracellular calcium in the brain: the plasma membrane Ca-ATPase and its regulatory protein, calmodulin. [14C]-labeled leucine was injected into seven month old adult Fischer 344 rats and the time-dependent appearance and loss of radioactivity was monitored in both the serum and proteins from the brains of rats sacrificed from 4 hours to 13 days after injection. Experimental data obtained for calmodulin and the plasma membrane Ca-ATPase are best described by theoretical curves accounting for leucine reutilization that assume apparent half-lives of 18 (± 2) hours and 12 (± 1) days, respectively.

Original languageEnglish (US)
Pages (from-to)163-165
Number of pages3
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Aug 8 1997

Bibliographical note

Funding Information:
This work was supported by grants from the American Federation for Aging Research for a Glenn/AFAR Scholarship to DAF and grants from the National Institute of Aging (R01 AG12275) and (P01 AG12993A).


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