Protein profiling employing capillary electrophoresis with dendrimers as pseudostationary phase media

Costas Stathakis, Edgar A. Arriaga, Norman J. Dovichi

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

The effects of anionic and cationic dendrimers as buffer additives on electropherograms of chicken sarcoplasmic proteins were examined. Starburst poly(amidoamine) dendrimers of generation G0 (cationic), 0.5, 1.5, 2.5 and 3.5 (anionic) were used in the concentration range of 10-5% to 1% g/ml. Anionic dendrimers, over the concentration range 10-5% to 0.1% g/ml, had a small effect on separation currents, which allows for tuning of selectivity and resolution over a wide range of dendrimer concentration with minimal contribution to buffer conductance and thus Joule heating. The resolution of protein electropherograms improved as a function of anionic dendrimer concentration and generation with a small effect on analysis times. A maximum of 10 components were identified in <10 min in the presence of 10-4% g/ml G2.5 dendrimer. Improved resolution was also observed in the presence of 0.001% g/ml cationic G0 as compared to buffers with no dendrimeric additives. Analysis times however were in excess of 40 min. Although improved protein profiles were obtained in the presence of dendrimers separation efficiencies however were quite low (13 000-76 000 theoretical plates). Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalJournal of Chromatography A
Volume817
Issue number1-2
DOIs
StatePublished - Aug 21 1998

Bibliographical note

Funding Information:
This work was supported by a Research Grant from the Natural Sciences and Engineering Research Council. Dr. Yung-Fong Cheng of Waters, is gratefully acknowledged for the generous donation of the Quanta 4000 instrument. R.J. Leblanc and E. LeBlanc of the Department of Agriculture, Food and Nutritional Science of the University of Alberta graciously provided the tissue samples. Special thanks to Darcy Driedger for his assistance with the MALDI-TOF measurements.

Keywords

  • Buffer composition
  • Dendrimers
  • Proteins

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