Abstract
The number of sequenced genomes is rapidly increasing, but functional annotation of the genes in these genomes lags far behind. Even in Arabidopsis (Arabidopsis thaliana), only approximately 40% of enzyme- and transporter-encoding genes have credible functional annotations, and this number is even lower in nonmodel plants. Functional characterization of unknown genes is a challenge, but various databases (e.g. for protein localization and coexpression) can be mined to provide clues. If homologous microbial genes exist—and about one-half the genes encoding unknown enzymes and transporters in Arabidopsis have microbial homologs—cross-kingdom comparative genomics can powerfully complement plant-based data. Multiple lines of evidence can strengthen predictions and warrant experimental characterization. In some cases, relatively quick tests in genetically tractable microbes can determine whether a prediction merits biochemical validation, which is costly and demands specialized skills.
Original language | English (US) |
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Pages (from-to) | 1436-1442 |
Number of pages | 7 |
Journal | Plant physiology |
Volume | 169 |
Issue number | 3 |
DOIs | |
State | Published - Nov 2015 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2015 American Society of Plant Biologists. All rights Reserved.