Proteolysis of the barley receptor-like protein kinase RPG1 by a proteasome pathway is correlated with Rpg1-mediated stem rust resistance

Jayaveeramuthu Nirmala, Stephanie Dahl, Brian J. Steffenson, C. Gamini Kannangara, Diter Von Wettstein, Xianming Chen, Andris Kleinhofs

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

In plants, disease resistance mediated by the gene-for-gene mechanism involves the recognition of specific effector molecules produced by the pathogen either directly or indirectly by the resistance-gene products. This recognition triggers a series of signals, thereby serving as a molecular switch in regulating defense mechanisms by the plants. To understand the mechanism of action of the barley stem rust resistance gene Rpg1, we investigated the fate of the RPG1 protein in response to infection with the stem rust fungus, Puccinia graminis f. sp. tritici. The investigations revealed that RPG1 disappears to undetectable limits only in the infected tissues in response to avirulent, but not virulent pathotypes. The RPG1 protein disappearance is rapid and appears to be due to specific protein degradation via the proteasome-mediated pathway as indicated by inhibition with the proteasomal inhibitor MG132, but not by other protease inhibitors.

Original languageEnglish (US)
Pages (from-to)10276-10281
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number24
DOIs
StatePublished - Jun 12 2007

Keywords

  • Avirulence
  • Cultivar
  • Programmed cell death
  • Puccinia graminis

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