TY - JOUR
T1 - Proteomic analysis of a mosquito host cell response to persistent Wolbachia infection
AU - Baldridge, Gerald
AU - Higgins, Lee Ann
AU - Witthuhn, Bruce
AU - Markowski, Todd
AU - Baldridge, Abigail
AU - Armien, Anibal
AU - Fallon, Ann
N1 - Publisher Copyright:
© 2017 Institut Pasteur
PY - 2017/9
Y1 - 2017/9
N2 - Wolbachia pipientis, an obligate intracellular bacterium associated with arthropods and filarial worms, is a target for filarial disease treatment and provides a gene drive agent for insect vector population suppression/replacement. We compared proteomes of Aedes albopictus mosquito C/wStr1 cells persistently infected with Wolbachia strain wStr, relative to uninfected C7-10 control cells. Among approximately 2500 proteins, iTRAQ data identified 815 differentially abundant proteins. As functional classes, energy and central intermediary metabolism proteins were elevated in infected cells, while suppressed proteins with roles in host DNA replication, transcription and translation suggested that Wolbachia suppresses pathways that support host cell growth and proliferation. Vacuolar ATPase subunits were strongly elevated, consistent with high densities of Wolbachia contained individually within vacuoles. Other differential level proteins had roles in ROS neutralization, protein modification/degradation and signaling, including hypothetical proteins whose functions in Wolbachia infection can potentially be manipulated by RNAi interference or transfection. Detection of flavivirus proteins supports further analysis of poorly understood, insect-specific flaviviruses and their potential interactions with Wolbachia, particularly in mosquitoes transinfected with Wolbachia. This study provides a framework for future attempts to manipulate pathways in insect cell lines that favor production of Wolbachia for eventual genetic manipulation, transformation and transinfection of vector species.
AB - Wolbachia pipientis, an obligate intracellular bacterium associated with arthropods and filarial worms, is a target for filarial disease treatment and provides a gene drive agent for insect vector population suppression/replacement. We compared proteomes of Aedes albopictus mosquito C/wStr1 cells persistently infected with Wolbachia strain wStr, relative to uninfected C7-10 control cells. Among approximately 2500 proteins, iTRAQ data identified 815 differentially abundant proteins. As functional classes, energy and central intermediary metabolism proteins were elevated in infected cells, while suppressed proteins with roles in host DNA replication, transcription and translation suggested that Wolbachia suppresses pathways that support host cell growth and proliferation. Vacuolar ATPase subunits were strongly elevated, consistent with high densities of Wolbachia contained individually within vacuoles. Other differential level proteins had roles in ROS neutralization, protein modification/degradation and signaling, including hypothetical proteins whose functions in Wolbachia infection can potentially be manipulated by RNAi interference or transfection. Detection of flavivirus proteins supports further analysis of poorly understood, insect-specific flaviviruses and their potential interactions with Wolbachia, particularly in mosquitoes transinfected with Wolbachia. This study provides a framework for future attempts to manipulate pathways in insect cell lines that favor production of Wolbachia for eventual genetic manipulation, transformation and transinfection of vector species.
KW - Aedes albopictus
KW - Flavivirus
KW - Intracellular bacterium
KW - Mosquito cell lines
KW - Transinfection
KW - Wolbachia pipientis
UR - http://www.scopus.com/inward/record.url?scp=85018963287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018963287&partnerID=8YFLogxK
U2 - 10.1016/j.resmic.2017.04.005
DO - 10.1016/j.resmic.2017.04.005
M3 - Article
C2 - 28435138
AN - SCOPUS:85018963287
SN - 0923-2508
VL - 168
SP - 609
EP - 625
JO - Research in Microbiology
JF - Research in Microbiology
IS - 7
ER -