Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells

Jiang Li, Chen Tan, Qiu Xiang, Xiaomei Zhang, Jian Ma, Jie ru Wang, Jianbo Yang, Wei fang Li, Shou rong Shen, Songping Liang, Guiyuan Li

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

In the previous study, we cloned a new gene, named NGX6, related to nasopharyngeal carcinoma (NPC) at 9p. To study its function in the pathogenesis of NPC, we have investigated changes in protein synthesis between NPC cell line HNE1 and that transfected with the gene. Using high-resolution two-dimensional electrophoresis, we found that 22 protein spots showed variations that were significant and reproducible. Analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches identified seven proteins that were upregulated and seven proteins that were downregulated. These proteins included Fas, zinc-finger protein (ZNF), RAB, and Ah receptor-interacting protein (AIP). The functional implications of the identified proteins are discussed.

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalJournal of Protein Chemistry
Volume20
Issue number3
DOIs
StatePublished - 2001

Keywords

  • Mass spectrometry
  • Protein identification
  • Two-dimensional gel electrophoresis

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