Protocol for examining human vaginal epithelial cell signaling in response to staphylococcal superantigens

Laura M. Breshears, Marnie L. Peterson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A detailed investigation of eukaryotic signaling pathways affected by bacterial products is key to our understanding of host-pathogen interactions. Cytokine expression appears to be an important initial host cell response to many bacterial products, including the Staphylococcus aureus superantigens (SAgs). While much is understood about how SAgs signal to immune cells, very little is known about the specifi c cellular pathways activated by SAgs on nonimmune cells such as those of the epithelium. Here, we describe methods for analyzing SAg signaling in cultured epithelial cells, which may be extrapolated to the analysis of signaling pathways induced by other bacterial ligands on a variety of cell types.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages149-158
Number of pages10
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1396
ISSN (Print)1064-3745

Keywords

  • Cell signaling
  • Ectodomain shedding
  • Epithelium
  • Mucosa
  • Staphylococcus aureus
  • Superantigen
  • Toxin

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