Psychophysical stress increases the expression of phospho-CREB, Fos protein and neurokinin-1 receptors in superficial laminae of trigeminal subnucleus caudalis in female rats

Sara L. Duenes, Randy Thompson, Zheng Chang, Keiichiro Okamoto, David A. Bereiter

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Psychological stress and estrogen status are risk factors to develop painful temporomandibular joint disorders (TMJD); however, the neural basis for this relationship is not known. This study tested the hypothesis that repeated forced swim stress and estradiol treatment alter the phosphorylation of cAMP responsive element-binding protein (pCREB) in trigeminal subnucleus caudalis (Vc), the initial site of sensory input from the TMJ. Ovariectomized female rats were given low or high dose estradiol and subjected to repeated forced swim stress for 3 days and on day 4 an intra-TMJ injection of mustard oil or vehicle was given. Forced swim alone increased the number of pCREB-positive neurons, independent of estradiol treatment or TMJ stimulation, in superficial and deep laminae of Vc. Forced swim also increased the number of Fos-positive neurons in superficial laminae and neurokinin-1 receptor mRNA in whole dorsal Vc, independent of estradiol treatment. These results indicated that persistent psychophysical stress alone was sufficient to increase the expression of pCREB and downstream regulated genes associated with enhanced excitability in the caudal medullary dorsal horn, a brainstem region thought to be critical for TMJD pain.

Original languageEnglish (US)
Pages (from-to)207-210
Number of pages4
JournalNeuroscience Letters
Volume486
Issue number3
DOIs
StatePublished - Dec 17 2010

Bibliographical note

Funding Information:
This study was supported by a grant from the National Institute of Dental and Craniofacial Research DE12758 and the Office of Research on Women's Health (ORWH) .

Keywords

  • Estradiol
  • Forced swim stress
  • Fos
  • Neurokinin-1 receptor
  • PCREB
  • Trigeminal subnucleus caudalis

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